The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene

L. Taraseviciene, A. Miczák, D. Apirion

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

A DNA clone complementing the rne-3071 mutation has been expressed and localized in the physical map of Escherichia coli. The DNA fragment from this clone was localized to the region of the E. coli chromosome where the rne-3071 mutation has been mapped. The position of this DNA fragment in the E. coli chromosome, the size of the product directed by this DNA fragment (110 000 Da), the restriction map of this fragment, the fact that the same clone complements the ems mutation, and the observation that the rne-3071 and the ams mutations cause similar patterns of RNA synthesis, show that the me gene - a gene specifying the processing endonuclease RNase E - and the ams gene - a gene that affects mRNA stability - are identical.

Original languageEnglish
Pages (from-to)851-855
Number of pages5
JournalMolecular Microbiology
Volume5
Issue number4
Publication statusPublished - Apr 1991

Fingerprint

RNA Stability
Mutation
Clone Cells
DNA
Escherichia coli
Genes
Chromosomes, Human, 16-18
Endonucleases
Chromosomes
RNA
ribonuclease E

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene. / Taraseviciene, L.; Miczák, A.; Apirion, D.

In: Molecular Microbiology, Vol. 5, No. 4, 04.1991, p. 851-855.

Research output: Contribution to journalArticle

@article{fe83082e17884c24bc49958222c3d9ed,
title = "The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene",
abstract = "A DNA clone complementing the rne-3071 mutation has been expressed and localized in the physical map of Escherichia coli. The DNA fragment from this clone was localized to the region of the E. coli chromosome where the rne-3071 mutation has been mapped. The position of this DNA fragment in the E. coli chromosome, the size of the product directed by this DNA fragment (110 000 Da), the restriction map of this fragment, the fact that the same clone complements the ems mutation, and the observation that the rne-3071 and the ams mutations cause similar patterns of RNA synthesis, show that the me gene - a gene specifying the processing endonuclease RNase E - and the ams gene - a gene that affects mRNA stability - are identical.",
author = "L. Taraseviciene and A. Micz{\'a}k and D. Apirion",
year = "1991",
month = "4",
language = "English",
volume = "5",
pages = "851--855",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene

AU - Taraseviciene, L.

AU - Miczák, A.

AU - Apirion, D.

PY - 1991/4

Y1 - 1991/4

N2 - A DNA clone complementing the rne-3071 mutation has been expressed and localized in the physical map of Escherichia coli. The DNA fragment from this clone was localized to the region of the E. coli chromosome where the rne-3071 mutation has been mapped. The position of this DNA fragment in the E. coli chromosome, the size of the product directed by this DNA fragment (110 000 Da), the restriction map of this fragment, the fact that the same clone complements the ems mutation, and the observation that the rne-3071 and the ams mutations cause similar patterns of RNA synthesis, show that the me gene - a gene specifying the processing endonuclease RNase E - and the ams gene - a gene that affects mRNA stability - are identical.

AB - A DNA clone complementing the rne-3071 mutation has been expressed and localized in the physical map of Escherichia coli. The DNA fragment from this clone was localized to the region of the E. coli chromosome where the rne-3071 mutation has been mapped. The position of this DNA fragment in the E. coli chromosome, the size of the product directed by this DNA fragment (110 000 Da), the restriction map of this fragment, the fact that the same clone complements the ems mutation, and the observation that the rne-3071 and the ams mutations cause similar patterns of RNA synthesis, show that the me gene - a gene specifying the processing endonuclease RNase E - and the ams gene - a gene that affects mRNA stability - are identical.

UR - http://www.scopus.com/inward/record.url?scp=0025881986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025881986&partnerID=8YFLogxK

M3 - Article

C2 - 1713282

AN - SCOPUS:0025881986

VL - 5

SP - 851

EP - 855

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 4

ER -