The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress

Verena Seidl, Bernhard Seiboth, L. Karaffa, Christian P. Kubicek

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39 Citations (Scopus)

Abstract

Fungi counteract extracellular osmotic pressure by producing intracellular polyols to prevent loss of water. In yeast osmotic signaling involves a MAP-kinase pathway culminating at the STRE-binding transcription factors Msn2/4. We investigated the role of a putative STRE-binding orthologue of Trichoderma atroviride, Seb1, in osmotic stress signaling. T. atroviride, subjected to osmotic stress (10% glucose or glycerol, 1 M KCl or NaCl), responds by raising its intracellular glycerol level. In contrast to Aspergillus nidulans, no erythritol is accumulated. Accumulation of glycerol levels under osmotic stress is strongly reduced in a seb1 deletion strain. To investigate glycerol biosynthesis in T. atroviride, the genes encoding glycerol dehydrogenase (gld1) and glycerol-3-phosphate dehydrogenase (gfd1) were cloned and characterized. Although both genes contain STRE-elements in their 5′-non-coding regions, only gld1 mRNA accumulates in response to osmotic stress, whereas expression of gfd1 remains at a constitutive level. In comparison to A. nidulans gld1 transcript levels in T. atroviride rise very slowly under conditions of salt stress. Deletion of seb1 results in a delayed accumulation of the gld1 transcript, but final levels match those in the wild-type whereas gfd1 transcript accumulation remains unaffected. Assays for glycerol dehydrogenase and glycerol-3-phosphate dehydrogenase enzymatic activities reveal an increase of the former-whereas the latter remains mainly unaffected-in the wild-type and the Δseb1 strain under different kinds of osmotic stress. The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4.

Original languageEnglish
Pages (from-to)1132-1140
Number of pages9
JournalFungal Genetics and Biology
Volume41
Issue number12
DOIs
Publication statusPublished - Dec 2004

Fingerprint

glycerol dehydrogenase
Trichoderma
Osmotic Pressure
Glycerol
Carrier Proteins
Gene Expression
Glycerolphosphate Dehydrogenase
Osmoregulation
Aspergillus nidulans
Yeasts
Erythritol
Genes
Fungi
Transcription Factors
Phosphotransferases
Salts
Glucose
Messenger RNA
Water

Keywords

  • gfd1
  • gld1
  • Glycerol
  • Glycerol dehydrogenase
  • Glycerol-3-phosphate dehydrogenase
  • Osmotic stress
  • Polyols
  • seb1
  • STRE-element
  • Trichoderma atroviride

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

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title = "The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress",
abstract = "Fungi counteract extracellular osmotic pressure by producing intracellular polyols to prevent loss of water. In yeast osmotic signaling involves a MAP-kinase pathway culminating at the STRE-binding transcription factors Msn2/4. We investigated the role of a putative STRE-binding orthologue of Trichoderma atroviride, Seb1, in osmotic stress signaling. T. atroviride, subjected to osmotic stress (10{\%} glucose or glycerol, 1 M KCl or NaCl), responds by raising its intracellular glycerol level. In contrast to Aspergillus nidulans, no erythritol is accumulated. Accumulation of glycerol levels under osmotic stress is strongly reduced in a seb1 deletion strain. To investigate glycerol biosynthesis in T. atroviride, the genes encoding glycerol dehydrogenase (gld1) and glycerol-3-phosphate dehydrogenase (gfd1) were cloned and characterized. Although both genes contain STRE-elements in their 5′-non-coding regions, only gld1 mRNA accumulates in response to osmotic stress, whereas expression of gfd1 remains at a constitutive level. In comparison to A. nidulans gld1 transcript levels in T. atroviride rise very slowly under conditions of salt stress. Deletion of seb1 results in a delayed accumulation of the gld1 transcript, but final levels match those in the wild-type whereas gfd1 transcript accumulation remains unaffected. Assays for glycerol dehydrogenase and glycerol-3-phosphate dehydrogenase enzymatic activities reveal an increase of the former-whereas the latter remains mainly unaffected-in the wild-type and the Δseb1 strain under different kinds of osmotic stress. The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4.",
keywords = "gfd1, gld1, Glycerol, Glycerol dehydrogenase, Glycerol-3-phosphate dehydrogenase, Osmotic stress, Polyols, seb1, STRE-element, Trichoderma atroviride",
author = "Verena Seidl and Bernhard Seiboth and L. Karaffa and Kubicek, {Christian P.}",
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T1 - The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress

AU - Seidl, Verena

AU - Seiboth, Bernhard

AU - Karaffa, L.

AU - Kubicek, Christian P.

PY - 2004/12

Y1 - 2004/12

N2 - Fungi counteract extracellular osmotic pressure by producing intracellular polyols to prevent loss of water. In yeast osmotic signaling involves a MAP-kinase pathway culminating at the STRE-binding transcription factors Msn2/4. We investigated the role of a putative STRE-binding orthologue of Trichoderma atroviride, Seb1, in osmotic stress signaling. T. atroviride, subjected to osmotic stress (10% glucose or glycerol, 1 M KCl or NaCl), responds by raising its intracellular glycerol level. In contrast to Aspergillus nidulans, no erythritol is accumulated. Accumulation of glycerol levels under osmotic stress is strongly reduced in a seb1 deletion strain. To investigate glycerol biosynthesis in T. atroviride, the genes encoding glycerol dehydrogenase (gld1) and glycerol-3-phosphate dehydrogenase (gfd1) were cloned and characterized. Although both genes contain STRE-elements in their 5′-non-coding regions, only gld1 mRNA accumulates in response to osmotic stress, whereas expression of gfd1 remains at a constitutive level. In comparison to A. nidulans gld1 transcript levels in T. atroviride rise very slowly under conditions of salt stress. Deletion of seb1 results in a delayed accumulation of the gld1 transcript, but final levels match those in the wild-type whereas gfd1 transcript accumulation remains unaffected. Assays for glycerol dehydrogenase and glycerol-3-phosphate dehydrogenase enzymatic activities reveal an increase of the former-whereas the latter remains mainly unaffected-in the wild-type and the Δseb1 strain under different kinds of osmotic stress. The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4.

AB - Fungi counteract extracellular osmotic pressure by producing intracellular polyols to prevent loss of water. In yeast osmotic signaling involves a MAP-kinase pathway culminating at the STRE-binding transcription factors Msn2/4. We investigated the role of a putative STRE-binding orthologue of Trichoderma atroviride, Seb1, in osmotic stress signaling. T. atroviride, subjected to osmotic stress (10% glucose or glycerol, 1 M KCl or NaCl), responds by raising its intracellular glycerol level. In contrast to Aspergillus nidulans, no erythritol is accumulated. Accumulation of glycerol levels under osmotic stress is strongly reduced in a seb1 deletion strain. To investigate glycerol biosynthesis in T. atroviride, the genes encoding glycerol dehydrogenase (gld1) and glycerol-3-phosphate dehydrogenase (gfd1) were cloned and characterized. Although both genes contain STRE-elements in their 5′-non-coding regions, only gld1 mRNA accumulates in response to osmotic stress, whereas expression of gfd1 remains at a constitutive level. In comparison to A. nidulans gld1 transcript levels in T. atroviride rise very slowly under conditions of salt stress. Deletion of seb1 results in a delayed accumulation of the gld1 transcript, but final levels match those in the wild-type whereas gfd1 transcript accumulation remains unaffected. Assays for glycerol dehydrogenase and glycerol-3-phosphate dehydrogenase enzymatic activities reveal an increase of the former-whereas the latter remains mainly unaffected-in the wild-type and the Δseb1 strain under different kinds of osmotic stress. The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4.

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KW - Trichoderma atroviride

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