The full length of a mitochondrial presequence is required for efficient monolayer insertion and interbilayer contact formation

Johanna M. Leenhouts, Z. Török, Rudy A. Demel, Johannes De Gier, Ben De Kruijff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Summary The peptide specificity of both presequence-monolayer interactions and the ability of presequences to induce interbilayer contacts between large unilamellar vesicles was investigated. A range of different synthetic peptides that are documented for their mitochondrial protein import abilities were used for this purpose. Both monolayer insertion and vesicle aggregation were found to be strongly dependent on the primary structure of the studied presequence peptides. The combination of monolayer data and results of vesicle aggregation experiments leads to the overall suggestion that monolayer insertion and interbilayer contact formation are mechanistically related. For maximal effects the full length of a presequence peptide is required. The cardiolipin specificity of presequence-induced interbilayer contact formation previously reported was found to be a more general property among presequence peptides. The peptide's ability to induce vesicle-vesicle contacts seems to parallel the efficiency of its import ability into mitochondria. These results lead to an extended hypothesis on the role of presequence-induced contact site formation during the mitochondrial protein import process.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalMolecular Membrane Biology
Volume11
Issue number3
DOIs
Publication statusPublished - 1994

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Peptides
Unilamellar Liposomes
Mitochondrial Proteins
Cardiolipins
Mitochondria

Keywords

  • Cardiolipin
  • Membrane contact
  • Mitochondrial presequence
  • Monolayer
  • Protein import

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The full length of a mitochondrial presequence is required for efficient monolayer insertion and interbilayer contact formation. / Leenhouts, Johanna M.; Török, Z.; Demel, Rudy A.; Gier, Johannes De; Kruijff, Ben De.

In: Molecular Membrane Biology, Vol. 11, No. 3, 1994, p. 159-164.

Research output: Contribution to journalArticle

Leenhouts, Johanna M. ; Török, Z. ; Demel, Rudy A. ; Gier, Johannes De ; Kruijff, Ben De. / The full length of a mitochondrial presequence is required for efficient monolayer insertion and interbilayer contact formation. In: Molecular Membrane Biology. 1994 ; Vol. 11, No. 3. pp. 159-164.
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