The Frequency of Peripheral Blood CD4+ CD25high FoxP3+ and CD4+ CD25- FoxP3+ Regulatory T Cells in Normal Pregnancy and Pre-Eclampsia

Gergely Toldi, Shigeru Saito, Tomoko Shima, Amrita Halmos, Zoltán Veresh, Barna Vásárhelyi, János Rigó, Attila Molvarec

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Problem: Regulatory T cells (Tregs) play an important role in the development of pregnancy-specific immune tolerance. We aimed to determine the peripheral frequency of a recently described Treg subpopulation, the CD4+ CD25- FoxP3+ Treg subset, and its correlation with the conventional CD4+ CD25high FoxP3+ Tregs in normal pregnancy (NP) and pre-eclampsia (PE) compared to non-pregnant (non-P) women. We also examined the proportion of the activated CD4+ CD25high FoxP3high Treg subset within conventional Treg cells. Method: We took peripheral blood samples from 20 PE, 20 NP, and 12 non-P women and determined the frequency of the above Treg subsets using flow cytometry. Results: The frequency of conventional CD4+ CD25high FoxP3+ Tregs and activated CD4+ CD25high FoxP3high Tregs, but also that of non-conventional CD4+ CD25- FoxP3+ Tregs was higher in NP compared to non-P women, but lower again in PE, reaching comparable levels to the non-P group. The ratios of CD4+ CD25high FoxP3+ and CD4+ CD25- FoxP3+ Treg subsets were constant in all three investigated groups. Conclusion: Our results indicate that the frequency of conventional and non-conventional Tregs alters simultaneously, and the presence in circulation of both of these Treg subsets is similarly important in the adequate development of pregnancy-specific immune tolerance.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume68
Issue number2
DOIs
Publication statusPublished - Aug 1 2012

Keywords

  • CD25
  • FoxP3
  • Pre-eclampsia
  • Pregnancy
  • Treg

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

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