The fragmentation of actin by thrombin. Isolation and characterization of the split products

L. Muszbek, J. A. Gladner, K. Laki

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Thrombin, a limited protease, hydrolyzes three bonds in actin at Arg-28, Arg-39, and Lys-113, thereby producing two smaller peptides and two larger fragments. The location of the bonds split was identified in the amino acid sequence of actin by isolating the split products and carrying out amino acid analysis, and N- and C-terminal determinations.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume167
Issue number1
DOIs
Publication statusPublished - 1975

Fingerprint

Thrombin
Actins
Amino Acids
Amino Acid Sequence
Peptide Hydrolases
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The fragmentation of actin by thrombin. Isolation and characterization of the split products. / Muszbek, L.; Gladner, J. A.; Laki, K.

In: Archives of Biochemistry and Biophysics, Vol. 167, No. 1, 1975, p. 99-103.

Research output: Contribution to journalArticle

@article{cacc7a8f903b42bcb49d79852d0bb7d6,
title = "The fragmentation of actin by thrombin. Isolation and characterization of the split products",
abstract = "Thrombin, a limited protease, hydrolyzes three bonds in actin at Arg-28, Arg-39, and Lys-113, thereby producing two smaller peptides and two larger fragments. The location of the bonds split was identified in the amino acid sequence of actin by isolating the split products and carrying out amino acid analysis, and N- and C-terminal determinations.",
author = "L. Muszbek and Gladner, {J. A.} and K. Laki",
year = "1975",
doi = "10.1016/0003-9861(75)90445-2",
language = "English",
volume = "167",
pages = "99--103",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The fragmentation of actin by thrombin. Isolation and characterization of the split products

AU - Muszbek, L.

AU - Gladner, J. A.

AU - Laki, K.

PY - 1975

Y1 - 1975

N2 - Thrombin, a limited protease, hydrolyzes three bonds in actin at Arg-28, Arg-39, and Lys-113, thereby producing two smaller peptides and two larger fragments. The location of the bonds split was identified in the amino acid sequence of actin by isolating the split products and carrying out amino acid analysis, and N- and C-terminal determinations.

AB - Thrombin, a limited protease, hydrolyzes three bonds in actin at Arg-28, Arg-39, and Lys-113, thereby producing two smaller peptides and two larger fragments. The location of the bonds split was identified in the amino acid sequence of actin by isolating the split products and carrying out amino acid analysis, and N- and C-terminal determinations.

UR - http://www.scopus.com/inward/record.url?scp=0016614461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016614461&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(75)90445-2

DO - 10.1016/0003-9861(75)90445-2

M3 - Article

C2 - 1130801

AN - SCOPUS:0016614461

VL - 167

SP - 99

EP - 103

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -