The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones

Szilárd Szikora, István Földi, Krisztina Tóth, Ede Migh, Andrea Vig, B. Bugyi, József Maléth, P. Hegyi, Péter Kaltenecker, Natalia Sanchez-Soriano, József Mihaly

Research output: Contribution to journalArticle

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Abstract

Directed axonal growth depends on correct coordination of the actin and microtubule cytoskeleton in the growth cone. However, despite the relatively large number of proteins implicated in actin-microtubule crosstalk, the mechanisms whereby actin polymerization is coupled to microtubule stabilization and advancement in the peripheral growth cone remained largely unclear. Here, we identified the formin Dishevelled-associated activator of morphogenesis (DAAM) as a novel factor playing a role in concerted regulation of actin and microtubule remodeling in Drosophila melanogaster primary neurons. In vitro, DAAM binds to F-actin as well as to microtubules and has the ability to crosslink the two filament systems. Accordingly, DAAM associates with the neuronal cytoskeleton, and a significant fraction of DAAM accumulates at places where the actin filaments overlap with that of microtubules. Loss of DAAM affects growth cone and microtubule morphology, and several aspects of microtubule dynamics; and biochemical and cellular assays revealed a microtubule stabilization activity and binding to the microtubule tip protein EB1. Together, these data suggest that, besides operating as an actin assembly factor, DAAM is involved in linking actin remodeling in filopodia to microtubule stabilization during axonal growth.

Original languageEnglish
Pages (from-to)2506-2519
Number of pages14
JournalJournal of Cell Science
Volume130
Issue number15
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Growth Cones
Actin Cytoskeleton
Morphogenesis
Microtubules
Actins
Microtubule Proteins
Pseudopodia
Growth
Drosophila melanogaster
Cytoskeleton
Polymerization
Neurons

Keywords

  • Actin
  • DAAM
  • Drosophila
  • Formin
  • Growth cone
  • Microtubule

ASJC Scopus subject areas

  • Cell Biology

Cite this

The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones. / Szikora, Szilárd; Földi, István; Tóth, Krisztina; Migh, Ede; Vig, Andrea; Bugyi, B.; Maléth, József; Hegyi, P.; Kaltenecker, Péter; Sanchez-Soriano, Natalia; Mihaly, József.

In: Journal of Cell Science, Vol. 130, No. 15, 01.08.2017, p. 2506-2519.

Research output: Contribution to journalArticle

Szikora, S, Földi, I, Tóth, K, Migh, E, Vig, A, Bugyi, B, Maléth, J, Hegyi, P, Kaltenecker, P, Sanchez-Soriano, N & Mihaly, J 2017, 'The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones', Journal of Cell Science, vol. 130, no. 15, pp. 2506-2519. https://doi.org/10.1242/jcs.203455
Szikora, Szilárd ; Földi, István ; Tóth, Krisztina ; Migh, Ede ; Vig, Andrea ; Bugyi, B. ; Maléth, József ; Hegyi, P. ; Kaltenecker, Péter ; Sanchez-Soriano, Natalia ; Mihaly, József. / The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones. In: Journal of Cell Science. 2017 ; Vol. 130, No. 15. pp. 2506-2519.
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