The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum

Erzsébet Baka, Sándor Varga, Cédric Hobel, Roland Wilhelm Knispel, Csaba Fekete, Milán Ivanics, Balázs Kriszt, István Nagy, József Kukolya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A transformation method yielding up to 104 transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH3.5 and 58°C. Shuttle vector maintenance could not be detected, since the used NovR gyraseB gene integrated into its chromosomal counterpart by homologous recombination.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalJournal of Microbiological Methods
Volume95
Issue number2
DOIs
Publication statusPublished - Nov 2013

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Keywords

  • Archaea
  • Homologous recombination
  • Novobiocin
  • Plasmid
  • Thermoplasma acidophilum
  • Transformation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Baka, E., Varga, S., Hobel, C., Knispel, R. W., Fekete, C., Ivanics, M., Kriszt, B., Nagy, I., & Kukolya, J. (2013). The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum. Journal of Microbiological Methods, 95(2), 145-148. https://doi.org/10.1016/j.mimet.2013.08.006