The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum

Erzsébet Baka, Sándor Varga, Cédric Hobel, Roland Wilhelm Knispel, C. Fekete, Milán Ivanics, Balázs Kriszt, István Nagy, József Kukolya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A transformation method yielding up to 104 transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH3.5 and 58°C. Shuttle vector maintenance could not be detected, since the used NovR gyraseB gene integrated into its chromosomal counterpart by homologous recombination.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalJournal of Microbiological Methods
Volume95
Issue number2
DOIs
Publication statusPublished - Nov 2013

Fingerprint

Thermoplasma
Archaea
Circular DNA
Genetic Vectors
Homologous Recombination
Maintenance
DNA
Genes

Keywords

  • Archaea
  • Homologous recombination
  • Novobiocin
  • Plasmid
  • Thermoplasma acidophilum
  • Transformation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum. / Baka, Erzsébet; Varga, Sándor; Hobel, Cédric; Knispel, Roland Wilhelm; Fekete, C.; Ivanics, Milán; Kriszt, Balázs; Nagy, István; Kukolya, József.

In: Journal of Microbiological Methods, Vol. 95, No. 2, 11.2013, p. 145-148.

Research output: Contribution to journalArticle

Baka, E, Varga, S, Hobel, C, Knispel, RW, Fekete, C, Ivanics, M, Kriszt, B, Nagy, I & Kukolya, J 2013, 'The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum', Journal of Microbiological Methods, vol. 95, no. 2, pp. 145-148. https://doi.org/10.1016/j.mimet.2013.08.006
Baka, Erzsébet ; Varga, Sándor ; Hobel, Cédric ; Knispel, Roland Wilhelm ; Fekete, C. ; Ivanics, Milán ; Kriszt, Balázs ; Nagy, István ; Kukolya, József. / The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum. In: Journal of Microbiological Methods. 2013 ; Vol. 95, No. 2. pp. 145-148.
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