The fate of nano-ZnO and its bulk counterpart in the body of microscopic nematodes: An X-ray spectrometric study

Zoltán Sávoly, Günter Buzanich, Giancarlo Pepponi, Christina Streli, Krisztina Hrács, Péter I. Nagy, G. Záray

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The distribution and speciation of zinc were studied in the body of female specimens of the soil-inhabiting, plant-feeding nematode, Xiphinema vuittenezi treated with nano-ZnO and its bulk counterpart. Lyophilized nematodes were studied by X-ray absorption near edge structure spectrometry (XANES) in order to characterize the zinc speciation. Furthermore, in the cross-sections prepared by focused ion beam technique, elemental maps were obtained using the electron probe microanalysis (EPMA) technique. XANES spectra were collected from three different regions (head, midbody and tail region) of nematodes in case of two different treatments (50mg/L bulk and nano-ZnO suspension, 24h long treatment). The sample spectra were fitted by the spectra of several reference compounds, the main components of the fitted spectra are the following in all cases: bulk/nano-ZnO; Zn-His and Zn3(PO4)2*xH2O. Consequently, partial biotransformation takes place for both treatments, histidine- and phosphate-rich ligands play dominant role in the binding of zinc. According to the result of the EPMA analysis, it can be established that the distribution of zinc correlates with the calcium and phosphorus distribution for both treatments. Considering the results of the investigations performed by the two microanalytical techniques, it is likely that calcium phosphate granules were formed in order to bind zinc and hereby to take part in zinc detoxification. Apparent difference in the behavior of bulk and nano-sized ZnO in the body of the investigated nematodes was not found.

Original languageEnglish
Pages (from-to)80-87
Number of pages8
JournalMicrochemical Journal
Volume118
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Zinc
X rays
X ray absorption
Electron probe microanalysis
Spectrometry
Detoxification
Focused ion beams
Histidine
Phosphorus
Suspensions
Phosphates
Ligands
Calcium
Soils

Keywords

  • Elemental distribution
  • Nematode
  • X-ray absorption near edge structure (XANES)
  • Zinc detoxification
  • ZnO nanoparticles

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

The fate of nano-ZnO and its bulk counterpart in the body of microscopic nematodes : An X-ray spectrometric study. / Sávoly, Zoltán; Buzanich, Günter; Pepponi, Giancarlo; Streli, Christina; Hrács, Krisztina; Nagy, Péter I.; Záray, G.

In: Microchemical Journal, Vol. 118, 01.01.2015, p. 80-87.

Research output: Contribution to journalArticle

Sávoly, Zoltán ; Buzanich, Günter ; Pepponi, Giancarlo ; Streli, Christina ; Hrács, Krisztina ; Nagy, Péter I. ; Záray, G. / The fate of nano-ZnO and its bulk counterpart in the body of microscopic nematodes : An X-ray spectrometric study. In: Microchemical Journal. 2015 ; Vol. 118. pp. 80-87.
@article{2ae5cb20246048688d49886c93bba037,
title = "The fate of nano-ZnO and its bulk counterpart in the body of microscopic nematodes: An X-ray spectrometric study",
abstract = "The distribution and speciation of zinc were studied in the body of female specimens of the soil-inhabiting, plant-feeding nematode, Xiphinema vuittenezi treated with nano-ZnO and its bulk counterpart. Lyophilized nematodes were studied by X-ray absorption near edge structure spectrometry (XANES) in order to characterize the zinc speciation. Furthermore, in the cross-sections prepared by focused ion beam technique, elemental maps were obtained using the electron probe microanalysis (EPMA) technique. XANES spectra were collected from three different regions (head, midbody and tail region) of nematodes in case of two different treatments (50mg/L bulk and nano-ZnO suspension, 24h long treatment). The sample spectra were fitted by the spectra of several reference compounds, the main components of the fitted spectra are the following in all cases: bulk/nano-ZnO; Zn-His and Zn3(PO4)2*xH2O. Consequently, partial biotransformation takes place for both treatments, histidine- and phosphate-rich ligands play dominant role in the binding of zinc. According to the result of the EPMA analysis, it can be established that the distribution of zinc correlates with the calcium and phosphorus distribution for both treatments. Considering the results of the investigations performed by the two microanalytical techniques, it is likely that calcium phosphate granules were formed in order to bind zinc and hereby to take part in zinc detoxification. Apparent difference in the behavior of bulk and nano-sized ZnO in the body of the investigated nematodes was not found.",
keywords = "Elemental distribution, Nematode, X-ray absorption near edge structure (XANES), Zinc detoxification, ZnO nanoparticles",
author = "Zolt{\'a}n S{\'a}voly and G{\"u}nter Buzanich and Giancarlo Pepponi and Christina Streli and Krisztina Hr{\'a}cs and Nagy, {P{\'e}ter I.} and G. Z{\'a}ray",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/j.microc.2014.08.011",
language = "English",
volume = "118",
pages = "80--87",
journal = "Microchemical Journal",
issn = "0026-265X",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - The fate of nano-ZnO and its bulk counterpart in the body of microscopic nematodes

T2 - An X-ray spectrometric study

AU - Sávoly, Zoltán

AU - Buzanich, Günter

AU - Pepponi, Giancarlo

AU - Streli, Christina

AU - Hrács, Krisztina

AU - Nagy, Péter I.

AU - Záray, G.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The distribution and speciation of zinc were studied in the body of female specimens of the soil-inhabiting, plant-feeding nematode, Xiphinema vuittenezi treated with nano-ZnO and its bulk counterpart. Lyophilized nematodes were studied by X-ray absorption near edge structure spectrometry (XANES) in order to characterize the zinc speciation. Furthermore, in the cross-sections prepared by focused ion beam technique, elemental maps were obtained using the electron probe microanalysis (EPMA) technique. XANES spectra were collected from three different regions (head, midbody and tail region) of nematodes in case of two different treatments (50mg/L bulk and nano-ZnO suspension, 24h long treatment). The sample spectra were fitted by the spectra of several reference compounds, the main components of the fitted spectra are the following in all cases: bulk/nano-ZnO; Zn-His and Zn3(PO4)2*xH2O. Consequently, partial biotransformation takes place for both treatments, histidine- and phosphate-rich ligands play dominant role in the binding of zinc. According to the result of the EPMA analysis, it can be established that the distribution of zinc correlates with the calcium and phosphorus distribution for both treatments. Considering the results of the investigations performed by the two microanalytical techniques, it is likely that calcium phosphate granules were formed in order to bind zinc and hereby to take part in zinc detoxification. Apparent difference in the behavior of bulk and nano-sized ZnO in the body of the investigated nematodes was not found.

AB - The distribution and speciation of zinc were studied in the body of female specimens of the soil-inhabiting, plant-feeding nematode, Xiphinema vuittenezi treated with nano-ZnO and its bulk counterpart. Lyophilized nematodes were studied by X-ray absorption near edge structure spectrometry (XANES) in order to characterize the zinc speciation. Furthermore, in the cross-sections prepared by focused ion beam technique, elemental maps were obtained using the electron probe microanalysis (EPMA) technique. XANES spectra were collected from three different regions (head, midbody and tail region) of nematodes in case of two different treatments (50mg/L bulk and nano-ZnO suspension, 24h long treatment). The sample spectra were fitted by the spectra of several reference compounds, the main components of the fitted spectra are the following in all cases: bulk/nano-ZnO; Zn-His and Zn3(PO4)2*xH2O. Consequently, partial biotransformation takes place for both treatments, histidine- and phosphate-rich ligands play dominant role in the binding of zinc. According to the result of the EPMA analysis, it can be established that the distribution of zinc correlates with the calcium and phosphorus distribution for both treatments. Considering the results of the investigations performed by the two microanalytical techniques, it is likely that calcium phosphate granules were formed in order to bind zinc and hereby to take part in zinc detoxification. Apparent difference in the behavior of bulk and nano-sized ZnO in the body of the investigated nematodes was not found.

KW - Elemental distribution

KW - Nematode

KW - X-ray absorption near edge structure (XANES)

KW - Zinc detoxification

KW - ZnO nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=84907494308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907494308&partnerID=8YFLogxK

U2 - 10.1016/j.microc.2014.08.011

DO - 10.1016/j.microc.2014.08.011

M3 - Article

AN - SCOPUS:84907494308

VL - 118

SP - 80

EP - 87

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

ER -