The eyes of transforming growth factor-β1 (TGF-β1) transgenic mice

Chi Chao Chan, Valentine Factor, Qian Li, Peter Nagy, Bo Peng, Snorri S. Thorgeirsson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Transforming growth factor-beta (TGFβ) is a potent regulator of cellular growth and immune function. The authors studied ocular histology and endotoxin-induced uveitis in a TGF-β1 transgenic (Tg) murine model. TGF-β1 Tg mice were generated by micro injecting a gene constructed by fusing the mouse albumin enhancer/promoter and porcine TGF-β1 cDNA. The eyes of Tg mice from two to 14 weeks of age were studied histologically. Tg mice, two to five weeks of age exhibited mild fragmentation of the lens fibers and retinal edema. No pathology was found from six to ten weeks of age, however, a progressive increased frequency of cataract was observed from 11 to 14 weeks of age. Plasma TGF-β1 levels were much higher in Tg mice than age-matched wild type control littermates (wt). Endotoxin-induced uveitis (EIU) in six- to eight-week-old Tg and wt mice was induced by footpad injection of lipopolysaccharide (LPS). Mice were euthanized 24 hr after LPS injection, the eyes were collected for histology and serum assayed for IL-6 and TGF-β1. There was a decrease in the mean numbers of infiltrating cells in Tg mice compared to wt mice. Serum IL-6 and TGF-β1 were much higher in Tg mice, The authors concluded that expression of the TGF-β1 transgene in the eyes may have effect on lens growth. Overexpression of TGF-β1 results in little or no effect on the development of EIU.

Original languageEnglish
Pages (from-to)183-191
Number of pages9
JournalOcular Immunology and Inflammation
Volume4
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

Keywords

  • Cataract
  • Endotoxin-induced uveitis (EIU)
  • Retinal edema
  • Transforming growth factor-beta 1 (TGF-β1)
  • Transgenic mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Ophthalmology

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