The extraction of Vitex agnus castus with different solvents

D. Cossuta, B. Simándi, E. Vági, J. Hohmann, T. Keve

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently more attention has been paid to healthy way of life, mainly the importance of healthy nutrition intake. First of all it means fibre-full and cholesterol-less nutrition intake, on the other hand solvent residual-free natural products are more favourable. The Supercritical Fluid Extraction (SFE) is one of the separation processes which produces solvent residual-free products, heat-sensitive compounds can be extracted and it is environmentally accepted technology. The goal of this work was to extract valuable components and natural agents from the fruit of chaste tree (Vitex agnus castus L.) Laboratory and pilot plant extractions were carried out using different solvents. For the laboratory Soxhlet extraction of Vitex agnus castus seeds four different solvents (n-hexane, ethyl-acetate, isopropyl-alcohol, ethyl-alcohol) were applied, all of them is permitted extraction solvents. Increasing the polarity of solvents higher extraction yields were achieved (n-hexane: 6.37 %; ethyl-acetate: 10.12 %; isopropyl-alcohol: 13.46 %; ethylalcohol: 18.11 %). Pilot plant experiments were performed in a Soxhlet extractor with ethyl-alcohol (96 % of purity) and in a 5 L volume high-pressure vessel with supercritical CO2 setting different pressure and temperature conditions. The pressure was changed from 100 bar up to 450 bar and the temperature was adjusted between 40-60°C. In one case 5 % ethyl-alcohol were added to the Sc-CO 2 as entrainer (at 300 bar and 40°C). The yields changed within a wide range (0.19 % - 9.97 %) according to the solvent power of the supercritical fluid. The extract samples were analised by dopamin D2 receptor binding assay, NMR, MS, and HPLC methods. Some compounds were isolated from the Agni casti fructus for the first time like vitetrifolin B, vitetrifolin C and eupatorin. Furthermore rotundifuran, vitexlactone, spathulenol, and two flavonoid derivatives were identified by NMR technology. The flavonoid derivatives were casticin and penduletin. Chaste tree extracts were examined in vitro antioxidant assay. This were an enzyme-dependent system of lipid peroxidation. The chaste tree extract is capable for treatment premenstrual syndrome (PMS). A product was developed which contains Vitex agnus castus extract as main constituent. A HPLC method was validated, which provides possibility to qualitative and quantitative determination of casticin from the extracts or the products originated from chaste tree.

Original languageEnglish
Title of host publicationCHISA 2006 - 17th International Congress of Chemical and Process Engineering
Publication statusPublished - 2006
EventCHISA 2006 - 17th International Congress of Chemical and Process Engineering - Prague, Czech Republic
Duration: Aug 27 2006Aug 31 2006

Other

OtherCHISA 2006 - 17th International Congress of Chemical and Process Engineering
CountryCzech Republic
CityPrague
Period8/27/068/31/06

Fingerprint

Flavonoids
Ethanol
2-Propanol
Nutrition
Hexane
Pilot plants
Assays
Alcohols
Nuclear magnetic resonance
Derivatives
Supercritical fluids
Cholesterol
Solvent extraction
Carbon Monoxide
Fruits
Antioxidants
Biological Products
Pressure vessels
Lipids
Seed

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Cossuta, D., Simándi, B., Vági, E., Hohmann, J., & Keve, T. (2006). The extraction of Vitex agnus castus with different solvents. In CHISA 2006 - 17th International Congress of Chemical and Process Engineering

The extraction of Vitex agnus castus with different solvents. / Cossuta, D.; Simándi, B.; Vági, E.; Hohmann, J.; Keve, T.

CHISA 2006 - 17th International Congress of Chemical and Process Engineering. 2006.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cossuta, D, Simándi, B, Vági, E, Hohmann, J & Keve, T 2006, The extraction of Vitex agnus castus with different solvents. in CHISA 2006 - 17th International Congress of Chemical and Process Engineering. CHISA 2006 - 17th International Congress of Chemical and Process Engineering, Prague, Czech Republic, 8/27/06.
Cossuta D, Simándi B, Vági E, Hohmann J, Keve T. The extraction of Vitex agnus castus with different solvents. In CHISA 2006 - 17th International Congress of Chemical and Process Engineering. 2006
Cossuta, D. ; Simándi, B. ; Vági, E. ; Hohmann, J. ; Keve, T. / The extraction of Vitex agnus castus with different solvents. CHISA 2006 - 17th International Congress of Chemical and Process Engineering. 2006.
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