The expressions of ABCC4 and ABCG2 xenobiotic transporters in human keratinocytes are proliferation-related

Attila Bebes, Kornélia Kis, Tünde Nagy, Anita Kurunczi, Hilda Polyánka, Z. Bata-Csörgő, L. Kemény, A. Dobozy, M. Széll

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Xenobiotic transporters of the ATP-binding cassette (ABC) protein superfamily play important roles in maintaining the biochemical barrier of various tissues, but their precise functions in the skin are not yet known. Screening of the expressions of the known xenobiotic transporter genes in two in vitro keratinocyte differentiation models revealed that the ABCC4 and ABCG2 transporters are highly expressed in proliferating keratinocytes, their expressions decreasing along with differentiation. Abrogation of the ABCC4 and ABCG2 protein functions by siRNA-mediated silencing and chemical inhibition did not affect the proliferation of HaCaT cells. In contrast, disruption of the ABCG2 function had no effect on normal human epidermal keratinocyte proliferation, while the inhibition of ABCC-type transporters by probenecid resulted in a striking decrease in the proliferation of the cells. These results indicate that, besides their possible therapy-modulating effects, xenobiotic transporters may contribute significantly to other keratinocyte functions, such as cell proliferation.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalArchives of Dermatological Research
Volume304
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Xenobiotics
Keratinocytes
Cell Proliferation
Probenecid
Small Interfering RNA
Carrier Proteins
Adenosine Triphosphate
Skin
ATP Binding Cassette Transporter, Sub-Family G, Member 2
Genes
Therapeutics

Keywords

  • Differentiation
  • Functional inhibition
  • Keratinocytes
  • Proliferation
  • Xenobiotic transporters

ASJC Scopus subject areas

  • Dermatology

Cite this

The expressions of ABCC4 and ABCG2 xenobiotic transporters in human keratinocytes are proliferation-related. / Bebes, Attila; Kis, Kornélia; Nagy, Tünde; Kurunczi, Anita; Polyánka, Hilda; Bata-Csörgő, Z.; Kemény, L.; Dobozy, A.; Széll, M.

In: Archives of Dermatological Research, Vol. 304, No. 1, 01.2012, p. 57-63.

Research output: Contribution to journalArticle

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