The expression strategy of goose parvovirus exhibits features of both the Dependovirus and Parvovirus genera

Jianming Qiu, Fang Cheng, Yuko Yoto, Z. Zádori, David Pintel

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The RNA transcription profile of the goose parvovirus (GPV) was determined, and it is a surprising hybrid of features of the Parvovirus and Dependovirus genera of the Parvovirinae subfamily of the Parvoviridae. Similar to the Dependovirus adeno-associated virus type 5, RNAs transcribed from the GPV upstream P9 promoter, which encode the viral nonstructural proteins, were polyadenylated at a high efficiency at a polyadenylation site [(pA)p] located within an intron in the center of the genome. Efficient usage of (pA)p required a downstream element that overlaps with the polypyrimidine tract of the A2 3′ splice site of the central intron. An upstream element required for efficient use of (pA)p was also identified. RNAs transcribed from the P42 promoter, presumed to encode the viral capsid proteins, primarily extended through (pA)p and were polyadenylated at a site, (pA)d, located at the right end of the genome and ultimately spliced at a high efficiency. No promoter analogous to the Dependovirus P19 promoter was detected; however, similar to minute virus of mice and other members of the Parvovirus genus, a significant portion of pre-mRNAs generated from the P9 promoter were additionally spliced within the putative GPV Rep1 coding region and likely encode an additional, smaller, nonstructural protein. Also similar to members of the Parvovirus genus, detectable activity of the GPV P42 promoter was highly dependent on transactivation by the GPV Rep1 protein in a manner dependent on binding to a cis-element located in the P42 promoter.

Original languageEnglish
Pages (from-to)11035-11044
Number of pages10
JournalJournal of Virology
Volume79
Issue number17
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Dependovirus
Geese
Parvovirus
Protoparvovirus
promoter regions
RNA
Parvovirinae
introns
Parvoviridae
Introns
Minute Virus of Mice
Viral Nonstructural Proteins
Genome
genome
viral proteins
RNA Splice Sites
Polyadenylation
transcriptional activation
Anseriform dependoparvovirus 1
coat proteins

ASJC Scopus subject areas

  • Immunology

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The expression strategy of goose parvovirus exhibits features of both the Dependovirus and Parvovirus genera. / Qiu, Jianming; Cheng, Fang; Yoto, Yuko; Zádori, Z.; Pintel, David.

In: Journal of Virology, Vol. 79, No. 17, 09.2005, p. 11035-11044.

Research output: Contribution to journalArticle

Qiu, Jianming ; Cheng, Fang ; Yoto, Yuko ; Zádori, Z. ; Pintel, David. / The expression strategy of goose parvovirus exhibits features of both the Dependovirus and Parvovirus genera. In: Journal of Virology. 2005 ; Vol. 79, No. 17. pp. 11035-11044.
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