The expression of several Cbf genes at the Fr-A2 locus is linked to frost resistance in wheat

Attila Vágújfalvi, Alessio Aprile, Andrea Miller, Jorge Dubcovsky, Giovanni Delugu, G. Galiba, Luigi Cattivelli

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

The C-repeat binding factor (Cbf) gene family has been shown to have a critical role in the regulation of low-temperature stress response in Arabidopsis. In Triticum monococcum, a locus carrying a family of Cbf-like genes, orthologs of Arabidopsis Cbf genes, is tightly linked to the frost tolerance locus Fr-Am 2, representing candidates for the differences in frost tolerance mapped at this locus. In this work we show that several Cbf genes have dramatically different levels of induction after cold exposure in hexaploid wheat. The Cbf-transcription levels differ between substitution and single chromosome recombinant lines carrying different 5A chromosomes or chromosome segments of the chromosome 5A from frost-tolerant and frost-sensitive wheat varieties. When the expression of eight Cbf genes, previously mapped at the Fr-A2 locus was investigated with gene specific primers using real-time RT-PCR, three Cbf sequences (Cbf1A, Cbf1C, Cbf7) showed a significantly higher relative transcription level (more than fourfold change) in lines differing for the Fr-A2 region. Differences in Cbf expression were also associated with a variation in frost tolerance. These results suggest that the amount of some Cbf mRNAs might be a critical factor for determining the level of frost tolerance in wheat.

Original languageEnglish
Pages (from-to)506-514
Number of pages9
JournalMolecular Genetics and Genomics
Volume274
Issue number5
DOIs
Publication statusPublished - Dec 2005

Fingerprint

varespladib methyl
Triticum
Chromosomes
Genes
Arabidopsis
Real-Time Polymerase Chain Reaction
Transcription Factors
Messenger RNA
Temperature

Keywords

  • Cbf
  • Frost tolerance
  • Transcription factor
  • Wheat

ASJC Scopus subject areas

  • Genetics

Cite this

The expression of several Cbf genes at the Fr-A2 locus is linked to frost resistance in wheat. / Vágújfalvi, Attila; Aprile, Alessio; Miller, Andrea; Dubcovsky, Jorge; Delugu, Giovanni; Galiba, G.; Cattivelli, Luigi.

In: Molecular Genetics and Genomics, Vol. 274, No. 5, 12.2005, p. 506-514.

Research output: Contribution to journalArticle

Vágújfalvi, A, Aprile, A, Miller, A, Dubcovsky, J, Delugu, G, Galiba, G & Cattivelli, L 2005, 'The expression of several Cbf genes at the Fr-A2 locus is linked to frost resistance in wheat', Molecular Genetics and Genomics, vol. 274, no. 5, pp. 506-514. https://doi.org/10.1007/s00438-005-0047-y
Vágújfalvi, Attila ; Aprile, Alessio ; Miller, Andrea ; Dubcovsky, Jorge ; Delugu, Giovanni ; Galiba, G. ; Cattivelli, Luigi. / The expression of several Cbf genes at the Fr-A2 locus is linked to frost resistance in wheat. In: Molecular Genetics and Genomics. 2005 ; Vol. 274, No. 5. pp. 506-514.
@article{f168c9d0166c442cb5f02bc4968ffd14,
title = "The expression of several Cbf genes at the Fr-A2 locus is linked to frost resistance in wheat",
abstract = "The C-repeat binding factor (Cbf) gene family has been shown to have a critical role in the regulation of low-temperature stress response in Arabidopsis. In Triticum monococcum, a locus carrying a family of Cbf-like genes, orthologs of Arabidopsis Cbf genes, is tightly linked to the frost tolerance locus Fr-Am 2, representing candidates for the differences in frost tolerance mapped at this locus. In this work we show that several Cbf genes have dramatically different levels of induction after cold exposure in hexaploid wheat. The Cbf-transcription levels differ between substitution and single chromosome recombinant lines carrying different 5A chromosomes or chromosome segments of the chromosome 5A from frost-tolerant and frost-sensitive wheat varieties. When the expression of eight Cbf genes, previously mapped at the Fr-A2 locus was investigated with gene specific primers using real-time RT-PCR, three Cbf sequences (Cbf1A, Cbf1C, Cbf7) showed a significantly higher relative transcription level (more than fourfold change) in lines differing for the Fr-A2 region. Differences in Cbf expression were also associated with a variation in frost tolerance. These results suggest that the amount of some Cbf mRNAs might be a critical factor for determining the level of frost tolerance in wheat.",
keywords = "Cbf, Frost tolerance, Transcription factor, Wheat",
author = "Attila V{\'a}g{\'u}jfalvi and Alessio Aprile and Andrea Miller and Jorge Dubcovsky and Giovanni Delugu and G. Galiba and Luigi Cattivelli",
year = "2005",
month = "12",
doi = "10.1007/s00438-005-0047-y",
language = "English",
volume = "274",
pages = "506--514",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - The expression of several Cbf genes at the Fr-A2 locus is linked to frost resistance in wheat

AU - Vágújfalvi, Attila

AU - Aprile, Alessio

AU - Miller, Andrea

AU - Dubcovsky, Jorge

AU - Delugu, Giovanni

AU - Galiba, G.

AU - Cattivelli, Luigi

PY - 2005/12

Y1 - 2005/12

N2 - The C-repeat binding factor (Cbf) gene family has been shown to have a critical role in the regulation of low-temperature stress response in Arabidopsis. In Triticum monococcum, a locus carrying a family of Cbf-like genes, orthologs of Arabidopsis Cbf genes, is tightly linked to the frost tolerance locus Fr-Am 2, representing candidates for the differences in frost tolerance mapped at this locus. In this work we show that several Cbf genes have dramatically different levels of induction after cold exposure in hexaploid wheat. The Cbf-transcription levels differ between substitution and single chromosome recombinant lines carrying different 5A chromosomes or chromosome segments of the chromosome 5A from frost-tolerant and frost-sensitive wheat varieties. When the expression of eight Cbf genes, previously mapped at the Fr-A2 locus was investigated with gene specific primers using real-time RT-PCR, three Cbf sequences (Cbf1A, Cbf1C, Cbf7) showed a significantly higher relative transcription level (more than fourfold change) in lines differing for the Fr-A2 region. Differences in Cbf expression were also associated with a variation in frost tolerance. These results suggest that the amount of some Cbf mRNAs might be a critical factor for determining the level of frost tolerance in wheat.

AB - The C-repeat binding factor (Cbf) gene family has been shown to have a critical role in the regulation of low-temperature stress response in Arabidopsis. In Triticum monococcum, a locus carrying a family of Cbf-like genes, orthologs of Arabidopsis Cbf genes, is tightly linked to the frost tolerance locus Fr-Am 2, representing candidates for the differences in frost tolerance mapped at this locus. In this work we show that several Cbf genes have dramatically different levels of induction after cold exposure in hexaploid wheat. The Cbf-transcription levels differ between substitution and single chromosome recombinant lines carrying different 5A chromosomes or chromosome segments of the chromosome 5A from frost-tolerant and frost-sensitive wheat varieties. When the expression of eight Cbf genes, previously mapped at the Fr-A2 locus was investigated with gene specific primers using real-time RT-PCR, three Cbf sequences (Cbf1A, Cbf1C, Cbf7) showed a significantly higher relative transcription level (more than fourfold change) in lines differing for the Fr-A2 region. Differences in Cbf expression were also associated with a variation in frost tolerance. These results suggest that the amount of some Cbf mRNAs might be a critical factor for determining the level of frost tolerance in wheat.

KW - Cbf

KW - Frost tolerance

KW - Transcription factor

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=28344440726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28344440726&partnerID=8YFLogxK

U2 - 10.1007/s00438-005-0047-y

DO - 10.1007/s00438-005-0047-y

M3 - Article

C2 - 16200412

AN - SCOPUS:28344440726

VL - 274

SP - 506

EP - 514

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 5

ER -