The Expression of Checkpoint and DNA Repair Genes in Head and Neck Cancer as Possible Predictive Factors

Orsolya Rusz, Margit Pál, Éva Szilágyi, László Rovó, Zoltán Varga, Bernadett Tomisa, Gabriella Fábián, Levente Kovács, Olga Nagy, Petra Mózes, Zita Reisz, L. Tiszlavicz, P. Deák, Z. Kahán

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

DNA damage response failure may influence the efficacy of DNA-damaging treatments. We determined the expression of 16 genes involved in distinct DNA damage response pathways, in association with the response to standard therapy. Twenty patients with locoregionally advanced, squamous cell head and neck carcinoma were enrolled. The treatment included induction chemotherapy (iChT) with docetaxel, cisplatin and 5-fluorouracil followed by concomitant chemoradiotherapy (ChRT) or radiotherapy (RT) alone. The volumetric metabolic therapeutic response was determined by [18F]FDG-PET/CT. In the tumor and matched normal tissues collected before treatment, the gene expressions were examined via the quantitative real-time polymerase chain reaction (qRT-PCR). The down-regulation of TP53 was apparently associated with a poor response to iChT, its up-regulation with complete regression in 2 cases. 7 cases with down-regulated REV1 expression showed complete regression after ChRT/RT, while 1 case with REV1 overexpression was resistant to RT. The overexpression of WRN was an independent predictor of tumor relapse. Our results suggest that an altered expression of REV1 predicts sensitivity to RT, while WRN overexpression is an unfavorable prognostic factor.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalPathology and Oncology Research
DOIs
Publication statusAccepted/In press - Jul 13 2016

Fingerprint

Head and Neck Neoplasms
DNA Repair
Radiotherapy
Induction Chemotherapy
Genes
docetaxel
Chemoradiotherapy
DNA Damage
Therapeutics
Gene Expression
Fluorodeoxyglucose F18
Fluorouracil
Cisplatin
Real-Time Polymerase Chain Reaction
Neoplasms
Up-Regulation
Down-Regulation
Recurrence
DNA

Keywords

  • Chemosensitivity
  • DNA damage response
  • Gene expression
  • Head and neck cancer
  • Radiosensitivity

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

The Expression of Checkpoint and DNA Repair Genes in Head and Neck Cancer as Possible Predictive Factors. / Rusz, Orsolya; Pál, Margit; Szilágyi, Éva; Rovó, László; Varga, Zoltán; Tomisa, Bernadett; Fábián, Gabriella; Kovács, Levente; Nagy, Olga; Mózes, Petra; Reisz, Zita; Tiszlavicz, L.; Deák, P.; Kahán, Z.

In: Pathology and Oncology Research, 13.07.2016, p. 1-12.

Research output: Contribution to journalArticle

Rusz, Orsolya ; Pál, Margit ; Szilágyi, Éva ; Rovó, László ; Varga, Zoltán ; Tomisa, Bernadett ; Fábián, Gabriella ; Kovács, Levente ; Nagy, Olga ; Mózes, Petra ; Reisz, Zita ; Tiszlavicz, L. ; Deák, P. ; Kahán, Z. / The Expression of Checkpoint and DNA Repair Genes in Head and Neck Cancer as Possible Predictive Factors. In: Pathology and Oncology Research. 2016 ; pp. 1-12.
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