The existence of lymphoid lineage restricted Philadelphia chromosome-positive acute lymphoblastic leukemia with heterogeneous bcr-abl rearrangement

L. Pajor, J. A. Vass, L. Kereskai, P. Kajtár, Á Szomor, M. Egyed, J. Iványi, P. Jáksó

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Analysis of lineage involvement was performed in 17 Philadelphia chromosome-positive acute lymphoblastic leukemia patients with no history of chronic myeloproliferative disorder. The percentage of blastic cells as defined by flow cytometry matched that of the Ph-positive cells in 14 out of 17 patients. The bcr-abl rearrangement was investigated by fluorescent in situ hybridization in morphologically identified blastic cells, myeloid elements, lymphocytes and erythroblasts using a combined light and fluorescent microscopical imaging. Lymphoid lineage restriction could be determined in all but three of the patients. These 14 patients exhibited heterogeneity in terms of m-bcr and M-bcr types of translocation as revealed by reverse transcription polymerase chain reaction. The three patients with multilineage involvement and M-bcr type of translocation reverted to chronic phase and the percentage of Ph-positive cells remained high. Thus, we could identify an uncommitted stem cell origin among Ph-positive ALLs only in those patients whose disease subsequently proved to be a lymphoid blastic crisis with clinically silent chronic phase.

Original languageEnglish
Pages (from-to)1122-1126
Number of pages5
JournalLeukemia
Volume14
Issue number6
Publication statusPublished - 2000

Fingerprint

Philadelphia Chromosome
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Myeloproliferative Disorders
Erythroblasts
Myeloid Cells
Fluorescence In Situ Hybridization
Reverse Transcription
Flow Cytometry
Stem Cells
Lymphocytes
Light
Polymerase Chain Reaction

Keywords

  • Lineage involvement
  • Lymphoblastic leukemia
  • Philadelphia chromosome

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

The existence of lymphoid lineage restricted Philadelphia chromosome-positive acute lymphoblastic leukemia with heterogeneous bcr-abl rearrangement. / Pajor, L.; Vass, J. A.; Kereskai, L.; Kajtár, P.; Szomor, Á; Egyed, M.; Iványi, J.; Jáksó, P.

In: Leukemia, Vol. 14, No. 6, 2000, p. 1122-1126.

Research output: Contribution to journalArticle

Pajor, L, Vass, JA, Kereskai, L, Kajtár, P, Szomor, Á, Egyed, M, Iványi, J & Jáksó, P 2000, 'The existence of lymphoid lineage restricted Philadelphia chromosome-positive acute lymphoblastic leukemia with heterogeneous bcr-abl rearrangement', Leukemia, vol. 14, no. 6, pp. 1122-1126.
Pajor, L. ; Vass, J. A. ; Kereskai, L. ; Kajtár, P. ; Szomor, Á ; Egyed, M. ; Iványi, J. ; Jáksó, P. / The existence of lymphoid lineage restricted Philadelphia chromosome-positive acute lymphoblastic leukemia with heterogeneous bcr-abl rearrangement. In: Leukemia. 2000 ; Vol. 14, No. 6. pp. 1122-1126.
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