The enzymes of acetyl-CoA metabolism in differentiating cholinergic (S-20) and noncholinergic (NIE-115) neuroblastoma cells

A. Szutowicz, M. R. Morrison, P. Srere

Research output: Contribution to journalArticle

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Abstract

Dibutyryl cyclic AMP and butyrate inhibited growth of S-20 (cholinergic) and NIE-115 (adrenergic) neuroblastoma clones. Both these drugs resulted in a parallel increase of choline acetyltransferase and ATP-citrate lyase activities in S-20 neuroblastoma cells. On the other hand, the increase in tyrosine hydroxylase activity in NIE-115 caused by these drugs was not accompanied by a significant change in ATP-citrate lyase activity. Both dibutyryl cyclic AMP and butyrate caused a decrease in fatty acid synthetase activity in both cell lines. The activities of pyruvate dehydrogenase, citrate synthase, choline acetyltransferase, and lactate dehydrogenase in both S-20 and NIE-115 cells were not significantly influenced by the drugs. ATP-citrate lyases from S-20 and NIE-115 had similar kinetic and immunological properties, and their subunits had the same molecular weight as the rat liver enzyme. These data indicate that the differential regulation of ATP-citrate lyase activity in cholinergic abd adrenergic cells does not result from the existence of different molecular forms of the enzyme in these cell lines. They also provide further evidence to support the hypothesis that ATP-citrate lyase activity increases during maturation of normal cholinergic neurons and decreases in noncholinergic cells of the brain.

Original languageEnglish
Pages (from-to)1664-1670
Number of pages7
JournalJournal of Neurochemistry
Volume40
Issue number6
Publication statusPublished - 1983

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ATP Citrate (pro-S)-Lyase
Acetyl Coenzyme A
Neuroblastoma
Metabolism
Cholinergic Agents
Enzymes
Bucladesine
Choline O-Acetyltransferase
Butyrates
Adrenergic Agents
Cells
Pharmaceutical Preparations
Cell Line
Citrate (si)-Synthase
Fatty Acid Synthases
Cholinergic Neurons
Tyrosine 3-Monooxygenase
Pyruvic Acid
L-Lactate Dehydrogenase
Liver

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

The enzymes of acetyl-CoA metabolism in differentiating cholinergic (S-20) and noncholinergic (NIE-115) neuroblastoma cells. / Szutowicz, A.; Morrison, M. R.; Srere, P.

In: Journal of Neurochemistry, Vol. 40, No. 6, 1983, p. 1664-1670.

Research output: Contribution to journalArticle

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