A 30-fold overexpression of the entire rne gene results in a 3-fold increase in RNase E activity. The overexpression of cloned rne fragments suggests that synthesis of the Rne protein is autoregulated and this regulation is related to the RNase E activity of the gene product. The enzyme produced by the cloned gene has an enhanced thermolability. The thermal stability of the temperature-sensitive enzyme increases when the cells are cultivated in the presence of chloramphenicol, which suggests posttranslational alterations.
|Number of pages||6|
|Journal||Acta microbiologica et immunologica Hungarica|
|Publication status||Published - 1995|
ASJC Scopus subject areas
- Immunology and Microbiology(all)