The endosymbiosis-induced genes ENOD40 and CCS52a are involved in endoparasitic-nematode interactions in Medicago truncatula

Bruno Favery, Arnaud Complainville, Jose Maria Vinardell, Philippe Lecomte, Danièle Vaubert, Peter Mergaert, Adam Kondorosi, É. Kondorosi, Martin Crespi, Pierre Abad

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Plants associate with a wide range of mutualistic and parasitic biotrophic organisms. Here, we investigated whether beneficial plant symbionts and biotrophic pathogens induce distinct or overlapping regulatory pathways in Medicago truncatula. The symbiosis between Sinorhizobium meliloti and this plant results in the formation of nitrogen-fixing root nodules requiring the activation of specific genes in the host plant. We studied expression patterns of nodule-expressed genes after infection with the root-knot nematode Meloidogyne incognita. Two regulators induced during nodule organogenesis, the early nodulin gene ENOD40 involved in primordium formation and the cell cycle gene CCS52a required for cell differentiation and endoreduplication, are expressed in galls of the host plant. Expression analysis of promoter-uidA fusions indicates an accumulation of CCS52a transcripts in giant cells undergoing endoreduplication, while ENOD40 expression is localized in surrounding cell layers. Transgenic plants overexpressing ENOD40 show a significantly higher number of galls. In addition, out of the 192 nodule-expressed genes tested, 38 genes were upregulated in nodules at least three-fold compared with control roots, but only two genes, nodulin 26 and cyclin D3, were found to be induced in galls. Taken together, these results suggest that certain events, such as endoreduplication, cell-to-cell communication with vascular tissues, or water transport, might be common between giant cell formation and nodule development.

Original languageEnglish
Pages (from-to)1008-1013
Number of pages6
JournalMolecular Plant-Microbe Interactions
Volume15
Issue number10
Publication statusPublished - Oct 1 2002

Fingerprint

Medicago truncatula
Symbiosis
symbiosis
Endoreduplication
Genes
Nematoda
endopolyploidy
galls
Giant Cells
nodulins
genes
giant cells
Plant Tumors
Cyclin D3
Tylenchoidea
Sinorhizobium meliloti
cdc Genes
Organogenesis
Genetically Modified Plants
host plants

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Favery, B., Complainville, A., Vinardell, J. M., Lecomte, P., Vaubert, D., Mergaert, P., ... Abad, P. (2002). The endosymbiosis-induced genes ENOD40 and CCS52a are involved in endoparasitic-nematode interactions in Medicago truncatula. Molecular Plant-Microbe Interactions, 15(10), 1008-1013.

The endosymbiosis-induced genes ENOD40 and CCS52a are involved in endoparasitic-nematode interactions in Medicago truncatula. / Favery, Bruno; Complainville, Arnaud; Vinardell, Jose Maria; Lecomte, Philippe; Vaubert, Danièle; Mergaert, Peter; Kondorosi, Adam; Kondorosi, É.; Crespi, Martin; Abad, Pierre.

In: Molecular Plant-Microbe Interactions, Vol. 15, No. 10, 01.10.2002, p. 1008-1013.

Research output: Contribution to journalArticle

Favery, B, Complainville, A, Vinardell, JM, Lecomte, P, Vaubert, D, Mergaert, P, Kondorosi, A, Kondorosi, É, Crespi, M & Abad, P 2002, 'The endosymbiosis-induced genes ENOD40 and CCS52a are involved in endoparasitic-nematode interactions in Medicago truncatula', Molecular Plant-Microbe Interactions, vol. 15, no. 10, pp. 1008-1013.
Favery B, Complainville A, Vinardell JM, Lecomte P, Vaubert D, Mergaert P et al. The endosymbiosis-induced genes ENOD40 and CCS52a are involved in endoparasitic-nematode interactions in Medicago truncatula. Molecular Plant-Microbe Interactions. 2002 Oct 1;15(10):1008-1013.
Favery, Bruno ; Complainville, Arnaud ; Vinardell, Jose Maria ; Lecomte, Philippe ; Vaubert, Danièle ; Mergaert, Peter ; Kondorosi, Adam ; Kondorosi, É. ; Crespi, Martin ; Abad, Pierre. / The endosymbiosis-induced genes ENOD40 and CCS52a are involved in endoparasitic-nematode interactions in Medicago truncatula. In: Molecular Plant-Microbe Interactions. 2002 ; Vol. 15, No. 10. pp. 1008-1013.
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