The endoderm and myocardium join forces to drive early heart tube assembly

Anastasiia Aleksandrova, A. Czirók, Edina Kosa, Oleksandr Galkin, Tracey J. Cheuvront, Brenda J. Rongish

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Formation of the muscular layer of the heart, the myocardium, involves the medial movement of bilateral progenitor fields; driven primarily by shortening of the endoderm during foregut formation. Using a combination of time-lapse imaging, microsurgical perturbations and computational modeling, we show that the speed of the medial-ward movement of the myocardial progenitors is similar, but not identical to that of the adjacent endoderm. Further, the extracellular matrix microenvironment separating the two germ layers also moves with the myocardium, indicating that collective tissue motion and not cell migration drives tubular heart assembly. Importantly, as myocardial cells approach the midline, they perform distinct anterior-directed movements relative to the endoderm. Based on the analysis of microincision experiments and computational models, we propose two characteristic, autonomous morphogenetic activities within the early myocardium: 1) an active contraction of the medial portion of the heart field and 2) curling- the tendency of the unconstrained myocardial tissue to form a spherical surface with a concave ventral side. In the intact embryo, these deformations are constrained by the endoderm and the adjacent mesoderm, nevertheless the corresponding mechanical stresses contribute to the proper positioning of myocardial primordia.

Original languageEnglish
Pages (from-to)40-54
Number of pages15
JournalDevelopmental Biology
Volume404
Issue number1
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Endoderm
Myocardium
Time-Lapse Imaging
Germ Layers
Mechanical Stress
Mesoderm
Cell Movement
Extracellular Matrix
Embryonic Structures

Keywords

  • Endoderm
  • Mechanics
  • Modeling
  • Myocardium

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Aleksandrova, A., Czirók, A., Kosa, E., Galkin, O., Cheuvront, T. J., & Rongish, B. J. (2015). The endoderm and myocardium join forces to drive early heart tube assembly. Developmental Biology, 404(1), 40-54. https://doi.org/10.1016/j.ydbio.2015.04.016

The endoderm and myocardium join forces to drive early heart tube assembly. / Aleksandrova, Anastasiia; Czirók, A.; Kosa, Edina; Galkin, Oleksandr; Cheuvront, Tracey J.; Rongish, Brenda J.

In: Developmental Biology, Vol. 404, No. 1, 01.08.2015, p. 40-54.

Research output: Contribution to journalArticle

Aleksandrova, A, Czirók, A, Kosa, E, Galkin, O, Cheuvront, TJ & Rongish, BJ 2015, 'The endoderm and myocardium join forces to drive early heart tube assembly', Developmental Biology, vol. 404, no. 1, pp. 40-54. https://doi.org/10.1016/j.ydbio.2015.04.016
Aleksandrova, Anastasiia ; Czirók, A. ; Kosa, Edina ; Galkin, Oleksandr ; Cheuvront, Tracey J. ; Rongish, Brenda J. / The endoderm and myocardium join forces to drive early heart tube assembly. In: Developmental Biology. 2015 ; Vol. 404, No. 1. pp. 40-54.
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