The effects of the urocortins on the hypothalamic-pituitary-adrenal axis - similarities and discordancies between rats and mice

Zsolt Bagosi, Krisztina Csabafi, Gergely Karasz, M. Jászberényi, I. Földesi, Andrea Siska, Gyula Szabó, G. Telegdy

Research output: Contribution to journalArticle

Abstract

The urocortins (Ucn I, Ucn II and Ucn III) are structural analogues of corticotropin-releasing factor (CRF). The aim of our present experiments was to compare the effects of the urocortins on the hypothalamic-pituitary-adrenal (HPA) axis in rats and mice, including the hypothalamic adrenocorticotropic hormone (ACTH) secretagogues, such as CRF and arginine vasopressin (AVP). Therefore, male CFLP mice and male Wistar rats were injected intracerebroventricularly (icv) with 0.5, 1, 2 and 5 μg/2 μl of Ucn I, Ucn II or Ucn III. After 30 min the animals were decapitated, and then, hypothalamic CRF and AVP concentrations and plasma ACTH and corticosterone (CORT) levels were measured. All measurements were performed by enzyme-linked immunosorbent assays (ELISA), except that of the plasma CORT level, which was determined by chemofluorescent assay. Ucn I increased significantly the hypothalamic CRF and AVP concentrations in both rats and mice. Ucn II and Ucn III influenced significantly only the hypothalamic CRF concentration in rats, without affecting the hypothalamic AVP concentration. In contrast, Ucn II and Ucn III increased significantly only the hypothalamic AVP concentration in mice, without affecting the hypothalamic CRF concentration. The hypothalamic changes were reflected more or less accurately by changes of the plasma ACTH and CORT levels. The present experiments demonstrate that the urocortins regulate the HPA axis centrally via modulation of the hypothalamic ACTH secretagogues and that there are some similarities and discordancies between rats and mice regarding this regulation.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalPeptides
Volume112
DOIs
Publication statusPublished - Feb 1 2019

Fingerprint

Urocortins
Rats
Corticotropin-Releasing Hormone
Arginine Vasopressin
Pituitary Hormone-Releasing Hormones
Adrenocorticotropic Hormone
Corticosterone
Hypothalamic Hormones
Plasmas
Assays
Immunosorbents
Wistar Rats
Animals

Keywords

  • HPA axis
  • Mice
  • Rats
  • Urocortins

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

The effects of the urocortins on the hypothalamic-pituitary-adrenal axis - similarities and discordancies between rats and mice. / Bagosi, Zsolt; Csabafi, Krisztina; Karasz, Gergely; Jászberényi, M.; Földesi, I.; Siska, Andrea; Szabó, Gyula; Telegdy, G.

In: Peptides, Vol. 112, 01.02.2019, p. 1-13.

Research output: Contribution to journalArticle

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