The effects of the operational parameters on the characteristics of protein microparticles

J. Tóth, Andrea Kardos-Fodor, Susan Halász-Péterfi, János Gyenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The aim of this study was to investigate the relation between the operational parameters of the salting-out precipitation and the particle size of the protein particles. For model material bovine serum albumin (BSA) was chosen which was salted out by different polyethylene glycols. Different solubilities and initial concentrations of the protein were chosen for the main operational parameters and the effect of the stirring and feeding conditions on particle size were also studied. In case of BSA precipitation there is a solubility change depending on the initial protein concentration which was also investigated. Beside the most deterministic process parameters on the demanded particle size (d̄ <10 μm) the attainable recovery yields were also taken into consideration. The precipitated protein was freeze-dried after separation, and the final product size was also compared to the operational parameters.

Original languageEnglish
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Publication statusPublished - 2006
Event2006 AIChE Spring National Meeting - 5th World Congress on Particle Technology - Orlando, FL, United States
Duration: Apr 23 2006Apr 27 2006

Other

Other2006 AIChE Spring National Meeting - 5th World Congress on Particle Technology
CountryUnited States
CityOrlando, FL
Period4/23/064/27/06

Fingerprint

Proteins
Particle size
Bovine Serum Albumin
Solubility
Polyethylene glycols
Recovery

Keywords

  • Bovine serum albumin
  • Polyethylene glycol
  • Precipitation

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Tóth, J., Kardos-Fodor, A., Halász-Péterfi, S., & Gyenis, J. (2006). The effects of the operational parameters on the characteristics of protein microparticles. In AIChE Annual Meeting, Conference Proceedings

The effects of the operational parameters on the characteristics of protein microparticles. / Tóth, J.; Kardos-Fodor, Andrea; Halász-Péterfi, Susan; Gyenis, János.

AIChE Annual Meeting, Conference Proceedings. 2006.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tóth, J, Kardos-Fodor, A, Halász-Péterfi, S & Gyenis, J 2006, The effects of the operational parameters on the characteristics of protein microparticles. in AIChE Annual Meeting, Conference Proceedings. 2006 AIChE Spring National Meeting - 5th World Congress on Particle Technology, Orlando, FL, United States, 4/23/06.
Tóth J, Kardos-Fodor A, Halász-Péterfi S, Gyenis J. The effects of the operational parameters on the characteristics of protein microparticles. In AIChE Annual Meeting, Conference Proceedings. 2006
Tóth, J. ; Kardos-Fodor, Andrea ; Halász-Péterfi, Susan ; Gyenis, János. / The effects of the operational parameters on the characteristics of protein microparticles. AIChE Annual Meeting, Conference Proceedings. 2006.
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