The effects of organic solvent/water mixtures on the structure and catalytic activity of porcine pepsin

L. M. Simon, M. Kotormán, A. Szabó, J. Nemcsók, I. Laczkó

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The effects of aqueous solutions of ethanol, acetonitrile and 1,4-dioxane in the concentration range 10-90% (v/v) on the activity of porcine pepsin were studied. The enzyme retained its activity in aqueous ethanol and aqueous acetonitrile with increasing organic solvent concentration up to 60%, and in aqueous 1,4-dioxane up to 30%, but thereafter a considerable decrease in activity was observed. The changes caused in the catalytic activity by the water-miscible organic solvents may be related to structural changes, which were followed by means of intrinsic fluorescence and circular dichroism spectroscopy measurements.

Original languageEnglish
Pages (from-to)909-912
Number of pages4
JournalProcess Biochemistry
Volume42
Issue number5
DOIs
Publication statusPublished - May 1 2007

Keywords

  • Aqueous organic solvents
  • CD spectroscopy
  • Enzyme activity
  • Intrinsic fluorescence
  • Porcine pepsin

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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