The effects of NaCl and some heavy metals on the denitrification activity of Ochrobactrum anthropi

Péter Kesserü, I. Kiss, Zoltán Bihari, Béla Polyák

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Ochrobactrum anthropi is a well-known Gram-negative bacterium, with the ability to degrade atrazine, urea-formaldehyde and chlorophenols. Investigation were made of the nitrate and nitrite reduction capacities of the strain in succinate and glucose media, and the tolerance of its denitrification to NaCl and some heavy metals. Succinate proved to be a better carbon source to drive denitrification by O. anthropi. Batch fermentation studies in anaerobic succinate medium indicated reduction capacities of 85.4 ± 9.1 and 48.6 ± 5.2 mgh-1g-1 dry cell for NO3- and NO2-, respectively. The nitrite accumulation of the cells revealed that O. anthropi is a group C denitrifying bacterium. Its growth in DSM 1 broth containing NaCl up to 40 g l-1 demonstrates that O. anthropi belongs in the group of moderately halophilic bacteria. Despite the fact that 42.5 g NaCl l-1 caused 50% growth inhibition in DSM 1 broth, the cells in the stationary phase readily tolerated NaCl concentrations up to 100 g l-1. Complete denitrification was achieved in test media containing 30 g NaCl l-1 after 1 week and the nitrate reductase retained its activity up to 100 g NaCl l-1. The cells were tolerant to Hg, Zn, Pb, Cu and Ni, and N2 was producted at tolerated concentrations of the metal in the cases of Hg and Pb.

Original languageEnglish
Pages (from-to)268-276
Number of pages9
JournalJournal of Basic Microbiology
Volume42
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Ochrobactrum anthropi
Denitrification
Heavy Metals
Succinic Acid
Nitrites
Chlorophenols
Bacteria
Atrazine
Nitrate Reductase
Growth
Gram-Negative Bacteria
Nitrates
Formaldehyde
Fermentation
Urea
Carbon
Metals
Glucose

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

The effects of NaCl and some heavy metals on the denitrification activity of Ochrobactrum anthropi. / Kesserü, Péter; Kiss, I.; Bihari, Zoltán; Polyák, Béla.

In: Journal of Basic Microbiology, Vol. 42, No. 4, 2002, p. 268-276.

Research output: Contribution to journalArticle

Kesserü, Péter ; Kiss, I. ; Bihari, Zoltán ; Polyák, Béla. / The effects of NaCl and some heavy metals on the denitrification activity of Ochrobactrum anthropi. In: Journal of Basic Microbiology. 2002 ; Vol. 42, No. 4. pp. 268-276.
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