The effects of female sexual hormones on the expression of aquaporin 5 in the late-pregnant rat uterus

Adrienn Csányi, Judit Bóta, G. Falkay, R. Gáspár, Eszter Ducza

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Thirteen mammalian aquaporin (AQP) water channels are known, and few of them play a role in the mammalian reproductive system. In our earlier study, the predominance of AQP5 in the late-pregnant rat uterus was proven. Our current aim was to investigate the effect of estrogen- and gestagen-related compounds on the expression of the AQP5 channel in the late-pregnant rat uterus. Furthermore, we examined the effect of hormonally-induced preterm delivery on the expression of AQP5 in the uterus. We treated pregnant Sprague-Dawley rats subcutaneously with 17β-estradiol, clomiphene citrate, tamoxifen citrate, progesterone, levonorgestrel, and medroxyprogesterone acetate. Preterm delivery was induced by subcutaneous mifepristone and intravaginal prostaglandin E2. Reverse-transcriptase PCR and Western blot techniques were used for the detection of the changes in AQP5 mRNA and protein expressions. The amount of AQP5 significantly increased after progesterone and progesterone analogs treatment on 18 and 22 days of pregnancy. The 17_-estradiol and estrogen receptor agonists did not influence the AQP5 mRNA level; however, estradiol induced a significant increase in the AQP5 protein level on the investigated days of gestation. Tamoxifen increased the AQP5 protein expression on day 18, while clomiphene citrate was ineffective. The hormonally-induced preterm birth significantly decreased the AQP5 level similarly to the day of delivery. We proved that AQP5 expression is influenced by both estrogen and progesterone in the late-pregnant rat uterus. The influence of progesterone on AQP5 expression is more predominant as compared with estrogen.

Original languageEnglish
Article number1300
JournalInternational Journal of Molecular Sciences
Volume17
Issue number8
DOIs
Publication statusPublished - Aug 22 2016

Fingerprint

Aquaporin 5
uterus
hormones
Hormones
rats
Uterus
Progesterone
estrogens
Rats
Estrogens
citrates
Proteins
Clomiphene
Aquaporins
Tamoxifen
delivery
Estradiol
proteins
reproductive systems
Estradiol Receptors

Keywords

  • Aquaporin 5
  • Estrogen
  • Gestagen
  • Pregnancy

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

The effects of female sexual hormones on the expression of aquaporin 5 in the late-pregnant rat uterus. / Csányi, Adrienn; Bóta, Judit; Falkay, G.; Gáspár, R.; Ducza, Eszter.

In: International Journal of Molecular Sciences, Vol. 17, No. 8, 1300, 22.08.2016.

Research output: Contribution to journalArticle

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