A duloxetin hatása a β-aktin stresszválaszra patkány agyban

Translated title of the contribution: The effects of duloxetine on β-actin stress response in rat brain

Szücs Szabina, Pákáski Magdolna, Domokos Ágnes, Kálmán János, Kálmán Sára, Garab Dénes, Penke Botond, Szabó Gyula, Janka Zoltán, Kálmán János

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Depression is a frequent prodromal symptom of Alzheimer's disease (AD). Stress factors play an important role in the etiopathology of both diseases, since increased corticosteroid levels caused by chronic stress indirectly induce neuronal damage. The aim of our experiments was to evaluate the changes induced by stress in the transcription of amyloid precursor protein (APP), mitogen activated protein kinase-1 (MAPK-1) and β-actin, of which the latest plays a leading role in synaptic plasticity. Additionally we intended to examine how duloxetine - a serotonin-norepinephrin reuptake inhibitor type antidepressant - would modify the stress-induced changes. Wistar rats were exposed to immobilization stress for five hours daily through 21 days, while part of the animals received 45 mg/bwkg of duloxetine. At the end of the third week total RNA was purified from the cortex and hippocampus. The amount of β-actin, APP and MAPK-1 mRNA was determined by real time PCR method. On protein level, semiquantitative measurement was performed by Western blot. The expression of β-actin mRNA in the animals exposed to stress was four times as intense as in the control group. The increase in the β-actin mRNA levels was repressed by the duloxetine treatment. In the case of APP and MAPK-1 no changes were detected. According to the Western blot results, the antidepressant treatment slightly, the drug along with the stress treatment strongly decreased the amount of the β-actin protein. Our findings indicate that antidepressant treatment with duloxetine could play a protective role against the chronic stress-induced changes in the nervous system, such as disorders of synaptic plasticity, and the consequent cognitive dysfunctions in case of both affective disorders and AD.

Translated title of the contributionThe effects of duloxetine on β-actin stress response in rat brain
Original languageHungarian
Pages (from-to)301-307
Number of pages7
JournalNeuropsychopharmacologia Hungarica
Volume12
Issue number1
Publication statusPublished - Mar 1 2010

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Neurology

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    Szabina, S., Magdolna, P., Ágnes, D., János, K., Sára, K., Dénes, G., Botond, P., Gyula, S., Zoltán, J., & János, K. (2010). A duloxetin hatása a β-aktin stresszválaszra patkány agyban. Neuropsychopharmacologia Hungarica, 12(1), 301-307.