The effect on bone regeneration of a liposomal vector to deliver BMP-2 gene to bone grafts in peri-implant bone defects

J. Park, R. Lutz, E. Felszeghy, J. Wiltfang, E. Nkenke, F. W. Neukam, K. A. Schlegel

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Successful bone-implant osseointegration in large peri-implant bone defects is often difficult, even through autologous bone grafting. Recently, cell-mediated regional gene therapy was introduced to deliver potent morphogens or growth factors in regenerative medicine. We applied liposomal vectors carrying bone morphogenetic protein (BMP)-2 cDNA directly into freshly created peri-implant bone defects on pig calvariae, with or without autologous bone graft. The BMP-2 gene was efficiently introduced into immigrating cells and trabecular cells lining the marginal bone surrounding the bony defect. After 1 week, abundant BMP-2 protein was detected throughout the peri-implant bone defect by immunohistochemistry. At 4 weeks, BMP-producing cells were still present in the defect and peri-implant area, which significantly enhanced new bone formation, compared with the control groups. Interestingly within a week of BMP-2 gene delivery with bone grafts, most osteoblastic cells lining the grafted bone chips also produced BMP-2. Particulated bone was immediately reorganized into newly formed trabecular bone. Grafted bone without BMP-2 gene delivery was still scattered and new bone matrix formation was not detected until 4 weeks after bone grafting. In conclusion, direct application of the BMP-2 gene using a liposomal vector enhanced bone regeneration in a bony defect and gene delivery combined with bone graft could induce a rapid osseointegration of the bone-implant interface at earlier stage.

Original languageEnglish
Pages (from-to)2772-2782
Number of pages11
JournalBiomaterials
Volume28
Issue number17
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Bone Morphogenetic Protein 2
Bone Regeneration
Grafts
Bone
Genes
Proteins
Transplants
Bone and Bones
Defects
Osseointegration
Bone Transplantation
Osteogenesis
Bone Morphogenetic Protein 4
Bone Matrix
Linings
Regenerative Medicine
Skull
Genetic Therapy
Intercellular Signaling Peptides and Proteins
Swine

Keywords

  • Bone graft
  • Bone morphogenetic protein (BMP)
  • De novo bone formation
  • Dental implant
  • Gene transfer
  • Liposome

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The effect on bone regeneration of a liposomal vector to deliver BMP-2 gene to bone grafts in peri-implant bone defects. / Park, J.; Lutz, R.; Felszeghy, E.; Wiltfang, J.; Nkenke, E.; Neukam, F. W.; Schlegel, K. A.

In: Biomaterials, Vol. 28, No. 17, 06.2007, p. 2772-2782.

Research output: Contribution to journalArticle

Park, J. ; Lutz, R. ; Felszeghy, E. ; Wiltfang, J. ; Nkenke, E. ; Neukam, F. W. ; Schlegel, K. A. / The effect on bone regeneration of a liposomal vector to deliver BMP-2 gene to bone grafts in peri-implant bone defects. In: Biomaterials. 2007 ; Vol. 28, No. 17. pp. 2772-2782.
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