The effect of zinc on iron-induced lipid peroxidation in different lipid systems including liposomes and micelles.

J. Szebeni, C. D. Eskelson, M. Chvapil

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The effect of zinc on FeSO4/ascorbic acid-induced lipid peroxidation was measured by the thiobarbituric acid assay in various lipid systems including small unilamellar liposomes prepared from egg phosphatidylcholine (EPC), ionic micelles prepared from arachidonic acid (C20:4), non-ionic monocomponent micelles prepared from EPC-derived, methylated fatty acids, and an eicosatetrene emulsion. With the exception of C20:4 micelles, zinc inhibited lipid peroxidation in each of the above systems in a similar dose-related fashion, with 0.5 mM zinc having maximal effect. Gas-chromatographic fatty acid analysis too indicated a protective effect of zinc against FeCl3-induced lipid peroxidation in soybean PC vesicles, which do not contain C20:4 moieties. These findings, in particular the inhibition of lipid peroxidation in eicosatetrene emulsion, suggest that the presence of uncharged polar head groups, or packing of lipid molecules into ordered self-assemblages (membranes and micelles) have no critical influence on the antioxidant effect of zinc. The results with Fe2+ are compatible with the concept that zinc interferes with the formation of Fe2+-oxygen-enoic complexes. This mechanism, however, cannot account for the inhibition by zinc of the Fe#+-induced lipid peroxidation, suggesting the involvement of other types of zinc effects in these systems.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalPhysiological Chemistry and Physics and Medical NMR
Volume20
Issue number3
Publication statusPublished - 1988

Fingerprint

Micelles
Liposomes
Lipid Peroxidation
Zinc
Iron
Lipids
Emulsions
Phosphatidylcholines
Ovum
Fatty Acids
Unilamellar Liposomes
Soybeans
Arachidonic Acid
Ascorbic Acid
Assays
Antioxidants
Gases
Oxygen
Membranes
Molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Physiology
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

The effect of zinc on iron-induced lipid peroxidation in different lipid systems including liposomes and micelles. / Szebeni, J.; Eskelson, C. D.; Chvapil, M.

In: Physiological Chemistry and Physics and Medical NMR, Vol. 20, No. 3, 1988, p. 205-211.

Research output: Contribution to journalArticle

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