The effect of vestibular nerve section on the expression of the hyaluronan in the frog, Rana esculenta

Gábor Halasi, Ervin Wolf, Tímea Bácskai, György Székely, László Módis, Zsuzsa M. Szigeti, Zoltán Mészár, Szabolcs Felszeghy, Clara Matesz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Following postganglionic lesion of the eighth cranial nerve, the changes in the expression of hyaluronan (HA), one of the extracellular matrix macromolecules, were examined in the medial (MVN) and lateral (LVN) vestibular nuclei and in the entry or transitional zone (TZ) of the nerve in the frog. HA was detected in different survival times by using a specific biotinylated hyaluronan-binding probe. HA expression was defined by the area-integrated optical density (AIOD), calculated from pixel intensities of digitally captured images. During the first postoperative days the perineuronal net (PN), a HA-rich area around the neurons, was not distinguishable from the surrounding neuropil in the MVN and LVN, characterized by a bilateral drop of AIOD specifically on the operated side. From postoperative day 14 onwards AIOD increased whilst the PN reorganized. In contrast, the AIOD wobbled up and down bilaterally without any trend in the TZ. Statistical analysis indicated that AIOD changes in the structures studied ran parallel bilaterally presumably because of the operation. Our results demonstrated for the first time that (1) the lesion of the eighth cranial nerve is accompanied by the modification of AIOD reflected HA expression in the MVN, LVN and TZ, (2) different tendencies exist in the time course of AIOD in the structures studied and (3) these tendencies are similar on the intact and operated sides. Our findings may suggest an area dependent molecular mechanism of HA in the restoration of vestibular function.

Original languageEnglish
Pages (from-to)321-334
Number of pages14
JournalBrain Structure and Function
Volume212
Issue number3-4
DOIs
Publication statusPublished - Dec 1 2007

Keywords

  • Brainstem
  • Extracellular matrix
  • Image analysis
  • Neural regeneration
  • Vestibular system

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience(all)
  • Histology

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