The effect of UV irradiation on uracil thin layer measured by optical waveguide lightmode spectroscopy

R. Horváth, T. Kerékgyártó, G. Csúcs, S. Gáspár, P. Illyés, G. Rontó, E. Papp

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The polycrystalline uracil thin-layer dosimeter is a well-established method to monitor the biological effects of the environmental ultraviolet (UV) radiation. It is based on the optical density (OD) decrease of the uracil layer in the UV absorption band due to photodimerization of the crystal caused by UV irradiation. In the present study, we report measurements made with optical waveguide lightmode spectroscopy (OWLS) to characterize the changes in the optogeometrical parameters of the uracil layer caused by an artificial UV source. It is shown that UV irradiation causes a decrease in the refractive index and an increase of the optical anisotropy. The determined kinetic parameters of the UV dose-sensor response curves correlate well with results of OD measurements, but the sensitivity of OWLS is about ten times higher. The results show that OWLS is capable of analyzing the UV response of the uracil layer and opens the way for dosimetrical applications.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalBiosensors and Bioelectronics
Volume16
Issue number1-2
DOIs
Publication statusPublished - 2001

Fingerprint

Uracil
Optical waveguides
Spectrum Analysis
Density (optical)
Irradiation
Spectroscopy
Optical anisotropy
Dosimeters
Kinetic parameters
Ultraviolet radiation
Refractometry
Absorption spectra
Refractive index
Anisotropy
Crystals
Sensors
Radiation

Keywords

  • Biological UV dosimetry
  • Optical parameters
  • OWLS
  • Uracil
  • UV radiation

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

The effect of UV irradiation on uracil thin layer measured by optical waveguide lightmode spectroscopy. / Horváth, R.; Kerékgyártó, T.; Csúcs, G.; Gáspár, S.; Illyés, P.; Rontó, G.; Papp, E.

In: Biosensors and Bioelectronics, Vol. 16, No. 1-2, 2001, p. 17-21.

Research output: Contribution to journalArticle

Horváth, R. ; Kerékgyártó, T. ; Csúcs, G. ; Gáspár, S. ; Illyés, P. ; Rontó, G. ; Papp, E. / The effect of UV irradiation on uracil thin layer measured by optical waveguide lightmode spectroscopy. In: Biosensors and Bioelectronics. 2001 ; Vol. 16, No. 1-2. pp. 17-21.
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