The effect of transmembrane potential on the dynamic behavior of cell membranes

Research output: Contribution to journalArticle

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Abstract

The relationship between transmembrane potential and lipid dynamics in the cytoplasmic membrane of mouse thymus cells has been investigated. Changes of transmembrane potential was followed by measuring the fluorescence emission of the anionic dye, bis-(1,3-dibutylbarbiturate)trimethine exonol (diBa-C4-(3)). Assessment of lipid fluidity was carried out applying three fluorescent lipid probes, 1-[4-(trimethylammonium)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH), 12-(9-anthroyloxy)stearic acid (12-AS) and 1,6-diphenyl-1,3,5-hexatriene (DPH) used to monitor different structural regions of the bilayer. The fluorescence anisotropy of these probes was measured as a function of temperature at two values of transmembrane potential. In the case of DPH it proved to depend on the membrane potential in the higher temperature range (above 28°C), while no such a dependence could be observed for DPH below this temperature range and for TMA-DPH and 12-AS in between 20 and 37°C. These data suggest that changes in transmembrane potential are accompanied with some local alteration in membrane lipid dynamics and/or structure.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalBBA - Biomembranes
Volume1023
Issue number1
DOIs
Publication statusPublished - Mar 30 1990

Fingerprint

Cell membranes
Membrane Potentials
Cell Membrane
Lipids
Temperature
Fluorescence
Diphenylhexatriene
Membranes
Thymus
Fluidity
Membrane Lipids
Fluorescence Polarization
Fluorescent Dyes
Anisotropy
Coloring Agents
Thymus Gland
1,3,5-hexatriene
diphenyl

Keywords

  • Cell membrane potential
  • Fluorescence
  • Fluorescence anisotropy
  • Lipid packing
  • Membrane fluidity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

The effect of transmembrane potential on the dynamic behavior of cell membranes. / Lakos, Z.; Somogyi, B.; Balázs, M.; Matkó, J.; Damjanovich, S.

In: BBA - Biomembranes, Vol. 1023, No. 1, 30.03.1990, p. 41-46.

Research output: Contribution to journalArticle

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