The effect of toxofilin on the structure and dynamics of monomeric actin

Lívia Czimbalek, Veronika Kollár, Roland Kardos, D. Lőrinczy, M. Nyitrai, G. Hild

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effects of toxofilin (an actin binding protein of Toxoplasma gondii) on G-actin was studied with spectroscopy techniques. Fluorescence anisotropy measurements proved that G-actin and toxofilin interact with 2:1 stoichiometry. The affinity of toxofilin to actin was also determined with a fluorescence anisotropy assay. Fluorescence quenching experiments showed that the accessibility of the actin bound ε-ATP decreased in the presence of toxofilin. The results can be explained by the shift of the nucleotide binding cleft into a closed conformational state. Differential scanning calorimetry measurements revealed that actin monomers become thermodynamically more stable due to the binding of toxofilin.

Original languageEnglish
Article number37348
Pages (from-to)3085-3089
Number of pages5
JournalFEBS Letters
Volume589
Issue number20
DOIs
Publication statusPublished - Oct 7 2015

Fingerprint

Actins
Fluorescence Polarization
Fluorescence
Anisotropy
Microfilament Proteins
Differential Scanning Calorimetry
Toxoplasma
Spectrum Analysis
Nucleotides
Stoichiometry
Differential scanning calorimetry
Quenching
Assays
Adenosine Triphosphate
Monomers
Spectroscopy
Experiments

Keywords

  • Actin
  • Calorimetry
  • Cytoskeleton
  • Dynamic
  • Fluorescence
  • Toxofilin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

The effect of toxofilin on the structure and dynamics of monomeric actin. / Czimbalek, Lívia; Kollár, Veronika; Kardos, Roland; Lőrinczy, D.; Nyitrai, M.; Hild, G.

In: FEBS Letters, Vol. 589, No. 20, 37348, 07.10.2015, p. 3085-3089.

Research output: Contribution to journalArticle

Czimbalek, Lívia ; Kollár, Veronika ; Kardos, Roland ; Lőrinczy, D. ; Nyitrai, M. ; Hild, G. / The effect of toxofilin on the structure and dynamics of monomeric actin. In: FEBS Letters. 2015 ; Vol. 589, No. 20. pp. 3085-3089.
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