The effect of toxins on the thermal stability of actin filaments by differential scanning calorimetry

B. Bugyi, G. Papp, Sz Halasi, B. Visegrády

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In our present study we performed the detailed characterisation of jasplakinolide and phalloidin on the thermal stability of actin filaments. The heat absorption curves were analysed by using the model established by Sanchez-Ruiz et al. [1]. The analysis provided the activation energies attributed to the heat denaturation of actin filaments in the absence and in the presence of toxins. The results indicated that there are kinetic differences between the toxin-mediated stabilization of the Ca2+-and Mg 2+-actin filaments. The effect of toxins appeared to be cation dependent.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalJournal of Thermal Analysis and Calorimetry
Volume82
Issue number1
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Actins
Differential scanning calorimetry
filaments
jasplakinolide
Thermodynamic stability
thermal stability
heat measurement
Denaturation
scanning
Phalloidine
heat
Stabilization
Activation energy
biopolymer denaturation
Positive ions
Kinetics
Cations
stabilization
activation energy
cations

Keywords

  • Actin
  • Cation
  • Differential scanning calorimetry
  • Jasplakinolide
  • Phalloidin
  • Protein conformation and dynamics

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

The effect of toxins on the thermal stability of actin filaments by differential scanning calorimetry. / Bugyi, B.; Papp, G.; Halasi, Sz; Visegrády, B.

In: Journal of Thermal Analysis and Calorimetry, Vol. 82, No. 1, 09.2005, p. 275-279.

Research output: Contribution to journalArticle

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