The effect of the short wavelength ultraviolet radiation. An extension of biological dosimetry to the UV-C range

G. Kovács, A. Fekete, A. Bérces, G. Rontó

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Polycrystalline uracil thin layer can be used as biological dosimeter for assessing exposure to UV radiation. The dimerization and reversion efficiency of the ultraviolet radiation in the UV-B and the UV-C range were quantified on polycrystalline uracil thin layers irradiated with quasi-monochromatic radiation using interference filters of 10 nm bandwidth. The dimer formation and monomerization (reversion) dose-effect relations were determined by optical spectroscopy. The decrease of the OD value of the uracil thin layer at 288 nm was taken as a measure of the dimer formation, while the increase of the OD of a completely irradiated (until reaching the saturation level) uracil layer was taken as the sign of the monomerization. The two processes in the UV-B and the UV-C range take place simultaneously, the individual characterization of the dimerization efficiency was performed from the initial slope of the dimerization dose-effect function and an action spectrum for dimerization was constructed in the UV-C range too. The reversion efficiency was found to be practically the same with all of the investigated wavelengths: 200 nm, 210 nm, 220 nm, 230 nm, 240 nm The possible biological relevance of the reversion of dimers are discussed.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume88
Issue number2-3
DOIs
Publication statusPublished - Sep 25 2007

Fingerprint

uracil
Uracil
Dimerization
dimerization
Ultraviolet radiation
ultraviolet radiation
Dosimetry
dosimeters
wavelengths
Dimers
Radiation
Wavelength
dimers
monochromatic radiation
dosage
Dosimeters
Spectrum Analysis
slopes
bandwidth
saturation

Keywords

  • Biological UV dosimetry
  • Nucleic acid damage
  • Photodimerization
  • Photomonomerization
  • Uracil thin layer
  • UV-C and UV-B radiation

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

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abstract = "Polycrystalline uracil thin layer can be used as biological dosimeter for assessing exposure to UV radiation. The dimerization and reversion efficiency of the ultraviolet radiation in the UV-B and the UV-C range were quantified on polycrystalline uracil thin layers irradiated with quasi-monochromatic radiation using interference filters of 10 nm bandwidth. The dimer formation and monomerization (reversion) dose-effect relations were determined by optical spectroscopy. The decrease of the OD value of the uracil thin layer at 288 nm was taken as a measure of the dimer formation, while the increase of the OD of a completely irradiated (until reaching the saturation level) uracil layer was taken as the sign of the monomerization. The two processes in the UV-B and the UV-C range take place simultaneously, the individual characterization of the dimerization efficiency was performed from the initial slope of the dimerization dose-effect function and an action spectrum for dimerization was constructed in the UV-C range too. The reversion efficiency was found to be practically the same with all of the investigated wavelengths: 200 nm, 210 nm, 220 nm, 230 nm, 240 nm The possible biological relevance of the reversion of dimers are discussed.",
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T1 - The effect of the short wavelength ultraviolet radiation. An extension of biological dosimetry to the UV-C range

AU - Kovács, G.

AU - Fekete, A.

AU - Bérces, A.

AU - Rontó, G.

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