The effect of the short wavelength ultraviolet radiation. An extension of biological dosimetry to the UV-C range

G. Kovács, A. Fekete, A. Bérces, Gy Rontó

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Polycrystalline uracil thin layer can be used as biological dosimeter for assessing exposure to UV radiation. The dimerization and reversion efficiency of the ultraviolet radiation in the UV-B and the UV-C range were quantified on polycrystalline uracil thin layers irradiated with quasi-monochromatic radiation using interference filters of 10 nm bandwidth. The dimer formation and monomerization (reversion) dose-effect relations were determined by optical spectroscopy. The decrease of the OD value of the uracil thin layer at 288 nm was taken as a measure of the dimer formation, while the increase of the OD of a completely irradiated (until reaching the saturation level) uracil layer was taken as the sign of the monomerization. The two processes in the UV-B and the UV-C range take place simultaneously, the individual characterization of the dimerization efficiency was performed from the initial slope of the dimerization dose-effect function and an action spectrum for dimerization was constructed in the UV-C range too. The reversion efficiency was found to be practically the same with all of the investigated wavelengths: 200 nm, 210 nm, 220 nm, 230 nm, 240 nm The possible biological relevance of the reversion of dimers are discussed.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalJournal of Photochemistry and Photobiology B: Biology
Issue number2-3
Publication statusPublished - Sep 25 2007



  • Biological UV dosimetry
  • Nucleic acid damage
  • Photodimerization
  • Photomonomerization
  • UV-C and UV-B radiation
  • Uracil thin layer

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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