The effect of the radioprotector WR-2721 and WR-1065 on mitochondrial lipid peroxidation

L. Tretter, E. Rónai, Gy Szabados, R. Hermann, A. Andó, I. Horváth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of mitochondrial enzymes. Both WR-1065 and glutathione were effective inhibitors of mitochondrial lipid peroxidation induced by ADP/Fe/NADPH or by ADP/Fe/ascorbate. Both thiols correspondingly delayed the free radical-mediated inactivation of succinate dehydrogenase and isocitrate dehydrogenase. WR-1065 was able to reduce cumene hydroperoxide non-enzymatically, and proved to be weak substrate for glutathione peroxidase. The disulfide formed from WR-1065 could be reduced by glutathione without the participation of glutathione reductase. A redox cycle is proposed between WR-1065, glutathione and glutathione reductase to explain the inhibitory effect of WR-1065 on lipid peroxidation.

Original languageEnglish
Pages (from-to)467-478
Number of pages12
JournalInternational Journal of Radiation Biology
Volume57
Issue number3
DOIs
Publication statusPublished - 1990

Fingerprint

Amifostine
glutathione
Lipids
Lipid Peroxidation
lipids
lipid peroxidation
glutathione-disulfide reductase
Glutathione
thiols
inactivation
Administrative data processing
adenosine diphosphate
dehydrogenases
Glutathione Reductase
isocitrate dehydrogenase
Sulfhydryl Compounds
deactivation
Adenosine Diphosphate
hydroperoxides
sulfides

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

The effect of the radioprotector WR-2721 and WR-1065 on mitochondrial lipid peroxidation. / Tretter, L.; Rónai, E.; Szabados, Gy; Hermann, R.; Andó, A.; Horváth, I.

In: International Journal of Radiation Biology, Vol. 57, No. 3, 1990, p. 467-478.

Research output: Contribution to journalArticle

Tretter, L. ; Rónai, E. ; Szabados, Gy ; Hermann, R. ; Andó, A. ; Horváth, I. / The effect of the radioprotector WR-2721 and WR-1065 on mitochondrial lipid peroxidation. In: International Journal of Radiation Biology. 1990 ; Vol. 57, No. 3. pp. 467-478.
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