Inappropriate vitamin and trace element supplementation may facilitate the development of atherosclerosis. It is known that Vitamin E protects lipids from oxidative stress, while clinical signs of atherosclerosis appear later in women compared to men. Aims: 1. The increase of vitamin E in plasma and plasma lipoproteins after 4 weeks of supplementation vitamin E was investigated, 2. furthermore it was tested whether a proportion shift occurs in α-tocopherol content of lipoproteins, 3. and checked for gender-related differences in plasma and plasma lipoprotein vitamin E levels before, during and after treatment, 4. plasma CRP levels as a marker of lipidperoxidation were also followed. Methods: 5-5 young healthy men and women took part in the study, receiving 700 IU/day Vitamin E for one month. Each subject was studied before and at the end of treatment, and also one month after treatment. HDL and LDL-VLDL containing lipoproteins were separated. Vitamin E and hsCRP levels were measured (by HPLC and an immunoturbidimetric method, respectively). Results: Vitamin E treatment induced in both genders an approximately threefold increase in vitamin E concentration in HDL-cholesterol (8.1 ± 1.7 μmol/l vs. 22.5 ± 7.5 μmol/l, p < 0.001), and a twofold increase in LDL-VLDL-cholesterol (22.0 ± 3.7 μmol/l vs. 49.0 ± 9.0 μmol/l, p < 0.001). Plasma and HDL vitamin E levels were higher in women than in men at the onset of treatment (6.8 ± 0.96 μmol/l vs. 9.5 ± 1.10 μmol/l), but during the treatment these gender-related differences disappeared. When plasma vitamin E concentration were considered 100% and the changes of the vitamin E concentrations of lipoproteins were calculated, it was found that supplementation with vitamin E in men increased the vitamin E concentration of LDL-VLDL cholesterol to a higher extent compared to women (LDL-VLDL % in men: 59.8 ± 7.43%, in women: 49.3 ± 7.41% p < 0.05). All the observed changes regressed one month after cessation of supplementation. The level of hsCRP decreased during vitamin E treatment (1.07 ± 0.9 mg/l vs. 0.2 ± 0.14 mg/l, p < 0.001), and remained suppressed after the cessation of treatment (0.37 ± 0.4, p < 0.01). Conclusions: These results support the hypothesis that women at young age are better protected against lipid-peroxidation as compared to men because of higher HDL vitamin E concentrations. Vitamin E supplementation in men eliminates this concentration difference between genders, and also increases LDL-VLDL vitamin E. In both genders high concentration of vitamin E in lipoproteins was associated with low hsCRP concentration.
|Translated title of the contribution||The effect of supplementary vitamin E on the vitamin content of lipoproteins in young men and women|
|Number of pages||6|
|Publication status||Published - Dec 1 2005|
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