The effect of substance P on the arachidonate cascade of rat platelets

B. Kis, Zsöfia Mezei, Á Geese, G. Telegdy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The role of substance P (SP) in neurogenic inflammation is well known. Through neurokinin receptors, SP activates cells, including the arachidonate cascade of platelets. Our in vitro experiments were carried out to determine the effect of SP on the arachidonate cascade of rat platelets. The platelets were labelled with 14C-arachidonic acid, and the 14C-eicosanoids were then separated by means of overpressure thin-layer chromatography or high-performance liquid chromatography and were quantitatively determined. SP (10-9 and 10-8) mol/L significantly increased the rate of the arachidonate cascade. The lipoxygenase pathway of platelets was stimulated by SP, which can result in the activation of protein kinase C mediated intracellular events. The cyclooxygenase system was inhibited by 10-12 mol/L, and stimulated by 10-9 mol/L SP. In our experiments SP in the physiological range of plasma concentration (10-12 mol/L) decreased the synthesis of vasoconstrictor arachidonate metabolites (TxA2 and PGF). These data suggest that in physiologic conditions the arachidonate cascade of platelets may play role in the vasodilator effect of SP. The formation of thromboxane in rat platelets was stimulated by higher concentration of SP (10-9 mol/L), and therefore the SP-induced cytotoxicity against parasites might be mediated by the stimulation of thromboxane A2 synthesis.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalActa Physiologica Hungarica
Volume85
Issue number2
Publication statusPublished - 1997

Fingerprint

Substance P
Blood Platelets
Neurogenic Inflammation
Neurokinin-1 Receptors
Thromboxane A2
Dinoprost
Lipoxygenase
Eicosanoids
Thromboxanes
Vasoconstrictor Agents
Prostaglandin-Endoperoxide Synthases
Thin Layer Chromatography
Vasodilator Agents
Arachidonic Acid
Protein Kinase C
Parasites
High Pressure Liquid Chromatography

Keywords

  • Platelet
  • Prostaglandin
  • Substance P
  • Thromboxane

ASJC Scopus subject areas

  • Physiology

Cite this

The effect of substance P on the arachidonate cascade of rat platelets. / Kis, B.; Mezei, Zsöfia; Geese, Á; Telegdy, G.

In: Acta Physiologica Hungarica, Vol. 85, No. 2, 1997, p. 89-97.

Research output: Contribution to journalArticle

Kis, B. ; Mezei, Zsöfia ; Geese, Á ; Telegdy, G. / The effect of substance P on the arachidonate cascade of rat platelets. In: Acta Physiologica Hungarica. 1997 ; Vol. 85, No. 2. pp. 89-97.
@article{cb9e2c27284947028b1d9ff90b7b4fcf,
title = "The effect of substance P on the arachidonate cascade of rat platelets",
abstract = "The role of substance P (SP) in neurogenic inflammation is well known. Through neurokinin receptors, SP activates cells, including the arachidonate cascade of platelets. Our in vitro experiments were carried out to determine the effect of SP on the arachidonate cascade of rat platelets. The platelets were labelled with 14C-arachidonic acid, and the 14C-eicosanoids were then separated by means of overpressure thin-layer chromatography or high-performance liquid chromatography and were quantitatively determined. SP (10-9 and 10-8) mol/L significantly increased the rate of the arachidonate cascade. The lipoxygenase pathway of platelets was stimulated by SP, which can result in the activation of protein kinase C mediated intracellular events. The cyclooxygenase system was inhibited by 10-12 mol/L, and stimulated by 10-9 mol/L SP. In our experiments SP in the physiological range of plasma concentration (10-12 mol/L) decreased the synthesis of vasoconstrictor arachidonate metabolites (TxA2 and PGF2α). These data suggest that in physiologic conditions the arachidonate cascade of platelets may play role in the vasodilator effect of SP. The formation of thromboxane in rat platelets was stimulated by higher concentration of SP (10-9 mol/L), and therefore the SP-induced cytotoxicity against parasites might be mediated by the stimulation of thromboxane A2 synthesis.",
keywords = "Platelet, Prostaglandin, Substance P, Thromboxane",
author = "B. Kis and Zs{\"o}fia Mezei and {\'A} Geese and G. Telegdy",
year = "1997",
language = "English",
volume = "85",
pages = "89--97",
journal = "Physiology International",
issn = "2498-602X",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - The effect of substance P on the arachidonate cascade of rat platelets

AU - Kis, B.

AU - Mezei, Zsöfia

AU - Geese, Á

AU - Telegdy, G.

PY - 1997

Y1 - 1997

N2 - The role of substance P (SP) in neurogenic inflammation is well known. Through neurokinin receptors, SP activates cells, including the arachidonate cascade of platelets. Our in vitro experiments were carried out to determine the effect of SP on the arachidonate cascade of rat platelets. The platelets were labelled with 14C-arachidonic acid, and the 14C-eicosanoids were then separated by means of overpressure thin-layer chromatography or high-performance liquid chromatography and were quantitatively determined. SP (10-9 and 10-8) mol/L significantly increased the rate of the arachidonate cascade. The lipoxygenase pathway of platelets was stimulated by SP, which can result in the activation of protein kinase C mediated intracellular events. The cyclooxygenase system was inhibited by 10-12 mol/L, and stimulated by 10-9 mol/L SP. In our experiments SP in the physiological range of plasma concentration (10-12 mol/L) decreased the synthesis of vasoconstrictor arachidonate metabolites (TxA2 and PGF2α). These data suggest that in physiologic conditions the arachidonate cascade of platelets may play role in the vasodilator effect of SP. The formation of thromboxane in rat platelets was stimulated by higher concentration of SP (10-9 mol/L), and therefore the SP-induced cytotoxicity against parasites might be mediated by the stimulation of thromboxane A2 synthesis.

AB - The role of substance P (SP) in neurogenic inflammation is well known. Through neurokinin receptors, SP activates cells, including the arachidonate cascade of platelets. Our in vitro experiments were carried out to determine the effect of SP on the arachidonate cascade of rat platelets. The platelets were labelled with 14C-arachidonic acid, and the 14C-eicosanoids were then separated by means of overpressure thin-layer chromatography or high-performance liquid chromatography and were quantitatively determined. SP (10-9 and 10-8) mol/L significantly increased the rate of the arachidonate cascade. The lipoxygenase pathway of platelets was stimulated by SP, which can result in the activation of protein kinase C mediated intracellular events. The cyclooxygenase system was inhibited by 10-12 mol/L, and stimulated by 10-9 mol/L SP. In our experiments SP in the physiological range of plasma concentration (10-12 mol/L) decreased the synthesis of vasoconstrictor arachidonate metabolites (TxA2 and PGF2α). These data suggest that in physiologic conditions the arachidonate cascade of platelets may play role in the vasodilator effect of SP. The formation of thromboxane in rat platelets was stimulated by higher concentration of SP (10-9 mol/L), and therefore the SP-induced cytotoxicity against parasites might be mediated by the stimulation of thromboxane A2 synthesis.

KW - Platelet

KW - Prostaglandin

KW - Substance P

KW - Thromboxane

UR - http://www.scopus.com/inward/record.url?scp=0031301515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031301515&partnerID=8YFLogxK

M3 - Article

C2 - 9706302

AN - SCOPUS:0031301515

VL - 85

SP - 89

EP - 97

JO - Physiology International

JF - Physiology International

SN - 2498-602X

IS - 2

ER -