The effect of stone-wool on rat lungs and on the primary culture of rat alveolar macrophages and type II pneumocytes

E. Tátrai, Márta Brozik, Ágnes Drahos, Zuzana Kováčiková, Éva Six, Márta Csík, Annamária Dám

Research output: Contribution to journalArticle

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Abstract

The effect of stone-wool has been studied in both in vivo long term sequential and in vitro methods in male Sprague-Dawley rats. Stone-wool was administered by single intratracheal instillation and the lungs were examined after 1, 3 and 6 months of exposure by morphological methods. UICC crocidolite was applied as a positive control. In addition, the effects of both fibres were examined in primary cultures of alveolar macrophages (AM) and type II pneumocytes (T2) by morphological, biochemical and immunological methods. By the end of 6 months stone-wool induced moderate pulmonary interstitial inflammation and fibrosis without progression, whereas crocidolite induced progressive interstitial inflammation and fibrosis as a function of time. Although stone-wool inhibited phagocytosis, it did not induce serious membrane damage to the cells examined and did not destroy their ultrastructure. It significantly reduced the activity of Cu,Zn/superoxide dismutase (SOD) and alkaline phosphatase (AP) in alveolar macrophages and significantly decreased the activity of AP and γ-glutamyl transpeptidase (GGT) in type II pneumocytes. Crocidolite, on the other hand, decreased the activity of all enzymes (glutathione peroxidase, GSH-Px; glutathione reductase, GSH-Rd) of glutathione metabolism as well as alkaline phosphatase in alveolar macrophages. It decreased the activity of all enzymes in type II pneumocytes, except for Cu,Zn/SOD. On exposure to stone-wool, the production of inflammatory proteins, macrophage chemoattractant protein-1 (MCP-1) and macrophage inhibitory protein-1α (MIP-1α) increased in both cultured cells but did not reach the level induced by crocidolite. Our results suggested that stone-wool is less toxic than crocidolite. Whether it is carcinogenic or not, is still an open question.

Original languageEnglish
Pages (from-to)16-24
Number of pages9
JournalJournal of Applied Toxicology
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Alveolar Epithelial Cells
Crocidolite Asbestos
Wool
Alveolar Macrophages
Rats
Lung
Alkaline Phosphatase
Macrophage Inflammatory Proteins
gamma-Glutamyltransferase
Glutathione Reductase
Pulmonary Fibrosis
Macrophages
Poisons
Chemotactic Factors
Enzymes
Glutathione Peroxidase
Phagocytosis
Metabolism
Glutathione
Sprague Dawley Rats

Keywords

  • Alveolar macrophages
  • Antioxidant system
  • EDXA
  • Lectins
  • MCP-1
  • MIP-1α
  • Pneumocytes
  • Pulmonary fibrosis
  • Stone-wool

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

The effect of stone-wool on rat lungs and on the primary culture of rat alveolar macrophages and type II pneumocytes. / Tátrai, E.; Brozik, Márta; Drahos, Ágnes; Kováčiková, Zuzana; Six, Éva; Csík, Márta; Dám, Annamária.

In: Journal of Applied Toxicology, Vol. 26, No. 1, 01.2006, p. 16-24.

Research output: Contribution to journalArticle

Tátrai, E. ; Brozik, Márta ; Drahos, Ágnes ; Kováčiková, Zuzana ; Six, Éva ; Csík, Márta ; Dám, Annamária. / The effect of stone-wool on rat lungs and on the primary culture of rat alveolar macrophages and type II pneumocytes. In: Journal of Applied Toxicology. 2006 ; Vol. 26, No. 1. pp. 16-24.
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