The effect of solution pH on the structural stability of magnetoferritin

L. Balejčíková, V. M. Garamus, M. V. Avdeev, V. I. Petrenko, L. Almásy, P. Kopčanský

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The structural stability of magnetoferritin, a synthetic analogue of ferritin, at various pH levels is assessed here. The structural and electrical properties of the complexes were determined by small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and zeta potential measurements. At pH 3 − 6 a reduction of electrostatic repulsion on the suspended colloids resulted in aggregation and sedimentation of magnetoferritin. At neutral to slightly alkaline conditions (pH 7–9) the magnetoferritin structure was stable for lower iron loadings. Higher solution pH 10–12 induced destabilization of the protein structure and dissociation of subunits. Increasing the loading factor in the MFer complex leads to decrease of the stability versus pH changes.

Original languageEnglish
Pages (from-to)375-381
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume156
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

structural stability
Dynamic light scattering
Zeta potential
Colloids
X ray scattering
Sedimentation
Structural properties
Electrostatics
Electric properties
Agglomeration
Ferritins
Iron
Proteins
destabilization
Static Electricity
colloids
light scattering
electrical properties
X-Rays
magnetoferritin

Keywords

  • Dynamic light scattering
  • Magnetoferritin solution
  • pH-dependent structure
  • Small-angle X-ray scattering
  • Structure and stability

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

The effect of solution pH on the structural stability of magnetoferritin. / Balejčíková, L.; Garamus, V. M.; Avdeev, M. V.; Petrenko, V. I.; Almásy, L.; Kopčanský, P.

In: Colloids and Surfaces B: Biointerfaces, Vol. 156, 01.08.2017, p. 375-381.

Research output: Contribution to journalArticle

Balejčíková, L. ; Garamus, V. M. ; Avdeev, M. V. ; Petrenko, V. I. ; Almásy, L. ; Kopčanský, P. / The effect of solution pH on the structural stability of magnetoferritin. In: Colloids and Surfaces B: Biointerfaces. 2017 ; Vol. 156. pp. 375-381.
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