The effect of sodium dodecyl sulfate and Pluronic F127 on the electrophoretic separation of protein and polypeptide test mixtures at acid pH

Ivan Mikšík, Adam Eckhardt, Esther Forgács, Tibor Cserháti, Zdeněk Deyl

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16 Citations (Scopus)

Abstract

Using a test mixture consisting of standard proteins (cytochrome c, chymotrypsinogen A, hen egg albumin, bovine serum albumin, aldolase, catalase and ferritin) and synthetic polypeptides (polylysine, polyaspartic, polyglutamic acid and polyproline) it was revealed that using sodium dodecyl sulfate (SDS) as background electrolyte modifier at acid pH (2.5) allows selective separation of highly positively charged polypeptides (polylysine) provided that their relative molecular mass is sufficiently low (3300 Da). The altered elution sequence of standard proteins as compared to a separation done without SDS may help their identification. Addition of Pluronic F127 offers clear-cut separations of standard proteins up to a relative molecular mass of 5 × 104 Da and allows to reveal protein/polypeptide microheterogeneity where applicable. None of the systems tested is suitable for the separation of acidic polypeptides and polyproline.

Original languageEnglish
Pages (from-to)1882-1886
Number of pages5
JournalELECTROPHORESIS
Volume23
Issue number12
DOIs
Publication statusPublished - Jul 22 2002

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Keywords

  • Capillary electrophoresis
  • Low pH
  • Peptide analysis
  • Pluronic F127

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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