The effect of simulated microgravity conditions on the TNF-alpha production by human PBMCS.

L. Batkai, A. Varkonyi, J. Mináróvits

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Our earlier space experiments demonstrated that the interferon production of human lymphocytes in microgravity is 4-8 times higher than those of the synchronous ground controls in vitro (Talas et al. 1983). These data suggested that the microgravity has a significant effect on cells. Since the possibilities to perform space-experiments are very limited and our study raised many interesting questions, we wished to simulate microgravity conditions in our laboratory. For this reason we purchased a Rotary Cell Culture System (RCCS) equipment to study different cell lines and human peripheral blood mononuclear cells (PBMCs) in experimental microgravity conditions. RCCS is a horizontally rotated bubble free culture vessel with membrane diffusion gas exchange. We report here an analysis of TNF-alpha (tumor necrosis factor-alpha) production by human PBMCs (control cultures exposed to simulated microgravity in RCCS). The cells were incubated in the presence or absence of either NDV (Newcastle Disease Virus) or one of the different forms (PHA-M or -P) of Phytohaemagglutinin.

Original languageEnglish
JournalJournal of gravitational physiology : a journal of the International Society for Gravitational Physiology
Volume6
Issue number1
Publication statusPublished - 1999

Fingerprint

Weightlessness
Tumor Necrosis Factor-alpha
Cell Culture Techniques
Blood Cells
Newcastle disease virus
Interferons
Gases
Lymphocytes
Cell Line
Equipment and Supplies
Membranes

Cite this

@article{23a4322c7ebd4c3e8c6d05869d6651ef,
title = "The effect of simulated microgravity conditions on the TNF-alpha production by human PBMCS.",
abstract = "Our earlier space experiments demonstrated that the interferon production of human lymphocytes in microgravity is 4-8 times higher than those of the synchronous ground controls in vitro (Talas et al. 1983). These data suggested that the microgravity has a significant effect on cells. Since the possibilities to perform space-experiments are very limited and our study raised many interesting questions, we wished to simulate microgravity conditions in our laboratory. For this reason we purchased a Rotary Cell Culture System (RCCS) equipment to study different cell lines and human peripheral blood mononuclear cells (PBMCs) in experimental microgravity conditions. RCCS is a horizontally rotated bubble free culture vessel with membrane diffusion gas exchange. We report here an analysis of TNF-alpha (tumor necrosis factor-alpha) production by human PBMCs (control cultures exposed to simulated microgravity in RCCS). The cells were incubated in the presence or absence of either NDV (Newcastle Disease Virus) or one of the different forms (PHA-M or -P) of Phytohaemagglutinin.",
author = "L. Batkai and A. Varkonyi and J. Min{\'a}r{\'o}vits",
year = "1999",
language = "English",
volume = "6",
journal = "Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology",
issn = "1077-9248",
publisher = "Galileo Foundation",
number = "1",

}

TY - JOUR

T1 - The effect of simulated microgravity conditions on the TNF-alpha production by human PBMCS.

AU - Batkai, L.

AU - Varkonyi, A.

AU - Mináróvits, J.

PY - 1999

Y1 - 1999

N2 - Our earlier space experiments demonstrated that the interferon production of human lymphocytes in microgravity is 4-8 times higher than those of the synchronous ground controls in vitro (Talas et al. 1983). These data suggested that the microgravity has a significant effect on cells. Since the possibilities to perform space-experiments are very limited and our study raised many interesting questions, we wished to simulate microgravity conditions in our laboratory. For this reason we purchased a Rotary Cell Culture System (RCCS) equipment to study different cell lines and human peripheral blood mononuclear cells (PBMCs) in experimental microgravity conditions. RCCS is a horizontally rotated bubble free culture vessel with membrane diffusion gas exchange. We report here an analysis of TNF-alpha (tumor necrosis factor-alpha) production by human PBMCs (control cultures exposed to simulated microgravity in RCCS). The cells were incubated in the presence or absence of either NDV (Newcastle Disease Virus) or one of the different forms (PHA-M or -P) of Phytohaemagglutinin.

AB - Our earlier space experiments demonstrated that the interferon production of human lymphocytes in microgravity is 4-8 times higher than those of the synchronous ground controls in vitro (Talas et al. 1983). These data suggested that the microgravity has a significant effect on cells. Since the possibilities to perform space-experiments are very limited and our study raised many interesting questions, we wished to simulate microgravity conditions in our laboratory. For this reason we purchased a Rotary Cell Culture System (RCCS) equipment to study different cell lines and human peripheral blood mononuclear cells (PBMCs) in experimental microgravity conditions. RCCS is a horizontally rotated bubble free culture vessel with membrane diffusion gas exchange. We report here an analysis of TNF-alpha (tumor necrosis factor-alpha) production by human PBMCs (control cultures exposed to simulated microgravity in RCCS). The cells were incubated in the presence or absence of either NDV (Newcastle Disease Virus) or one of the different forms (PHA-M or -P) of Phytohaemagglutinin.

UR - http://www.scopus.com/inward/record.url?scp=0033155892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033155892&partnerID=8YFLogxK

M3 - Article

VL - 6

JO - Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology

JF - Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology

SN - 1077-9248

IS - 1

ER -