The effect of SH-SS transition in the structural organization of mitotic chromosomes

G. Kosztolányi, Erica M. Bühler

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human lymphoid cell cultures were treated with 2,2′-dithiodipyridine, a thiol reacting agent which produces SS bridges inside the living cells. After 1 to 4h treatment chromosome preparations were made. The chromosomes acquired a peculiar segmentation along the chromatids. The results suggest that the alteration might arise from a direct reaction of the agent with special chromosomal proteins. The resistance of treated chromosomes to alkali-heat treatment used in C-banding technique was different from that of untreated chromosomes. This prefixational procedure seems to be advantageous in analysing both the chromosome organization and the banding mechanism.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalHuman Genetics
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 1978

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Chromosomes
Chromosome Banding
Chromatids
Alkalies
Sulfhydryl Compounds
Cell Culture Techniques
Hot Temperature
Lymphocytes
Proteins

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

The effect of SH-SS transition in the structural organization of mitotic chromosomes. / Kosztolányi, G.; Bühler, Erica M.

In: Human Genetics, Vol. 42, No. 1, 01.1978, p. 83-88.

Research output: Contribution to journalArticle

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