The effect of pH on the thermal stability of α-actin isoforms

G. Papp, B. Bugyi, Z. Ujfalusi, Sz Halasi, J. Orbán

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of pH was characterised on the thermal stability of magnesium saturated skeletal and cardiac α-actin isoforms with differential scanning calorimetry (DSC) at pH 7.0 and 8.0. The calorimetric curves were further analysed to calculate the enthalpy and transition entropy changes. The activation energy was also determined to describe the energy consumption of the initiation of the thermal denaturation process. Although the difference in T mvalues is too small to interpret the difference between the a-actin isoforms, the values of the activation energy indicated that the α-skeletal actin is probably more stable compared to the α-cardiac actin. The difference in the activation energies indicated that lowering the pH can produce a more stable protein matrix in both cases of the isoforms. The larger range of the difference in the values of the activation energies suggested that the α-cardiac actin is probably more sensitive to the change of the pH compared to the α -skeletal actin.

Original languageEnglish
Pages (from-to)281-285
Number of pages5
JournalJournal of Thermal Analysis and Calorimetry
Volume82
Issue number1
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Actins
Protein Isoforms
Thermodynamic stability
thermal stability
Activation energy
activation energy
Denaturation
biopolymer denaturation
energy consumption
Magnesium
magnesium
Differential scanning calorimetry
Enthalpy
Entropy
heat measurement
Energy utilization
enthalpy
entropy
proteins
Proteins

Keywords

  • Actin isoform
  • Activation energy
  • Calorimetry
  • Divalent cation
  • pH

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

The effect of pH on the thermal stability of α-actin isoforms. / Papp, G.; Bugyi, B.; Ujfalusi, Z.; Halasi, Sz; Orbán, J.

In: Journal of Thermal Analysis and Calorimetry, Vol. 82, No. 1, 09.2005, p. 281-285.

Research output: Contribution to journalArticle

Papp, G. ; Bugyi, B. ; Ujfalusi, Z. ; Halasi, Sz ; Orbán, J. / The effect of pH on the thermal stability of α-actin isoforms. In: Journal of Thermal Analysis and Calorimetry. 2005 ; Vol. 82, No. 1. pp. 281-285.
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