Abstract
High resolution NMR imaging of the isolated perfused rat hippocampal slice was used to quantitate ADC changes following ouabain-induced cell swelling. Hippocampal slices were studied in artificial cerebrospinal fluid and then in ouabain using a 600-MHz narrow bore spectrometer and a home- built perfusion chamber. The brain slices demonstrated biexponential diffusion behavior. After perfusion with 1 mM ouabain, there was an increase in the fraction of slowly diffusing water. The ADCs of the two fractions did not change. These data support the hypothesis that the decrease in the ADC of brain water following an ischemic attack is caused by cell swelling. The relative amplitudes of the two diffusing fractions do not match the expected ratio of intracellular and extracellular fractions. This discrepancy may be principally due to the difference in T2 relaxation rates of the two compartments.
| Original language | English |
|---|---|
| Pages (from-to) | 137-142 |
| Number of pages | 6 |
| Journal | Magnetic Resonance in Medicine |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1999 |
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Keywords
- Ischemia
- NMR imaging
- Ouabain
- Water diffusion
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
Cite this
The effect of ouabain on water diffusion in the rat hippocampal slice measured by high resolution NMR imaging. / Buckley, David L.; Bui, Jonathan D.; Phillips, M. Ian; Zelles, T.; Inglis, Benjamin A.; Plant, H. Daniel; Blackband, Stephen J.
In: Magnetic Resonance in Medicine, Vol. 41, No. 1, 1999, p. 137-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of ouabain on water diffusion in the rat hippocampal slice measured by high resolution NMR imaging
AU - Buckley, David L.
AU - Bui, Jonathan D.
AU - Phillips, M. Ian
AU - Zelles, T.
AU - Inglis, Benjamin A.
AU - Plant, H. Daniel
AU - Blackband, Stephen J.
PY - 1999
Y1 - 1999
N2 - High resolution NMR imaging of the isolated perfused rat hippocampal slice was used to quantitate ADC changes following ouabain-induced cell swelling. Hippocampal slices were studied in artificial cerebrospinal fluid and then in ouabain using a 600-MHz narrow bore spectrometer and a home- built perfusion chamber. The brain slices demonstrated biexponential diffusion behavior. After perfusion with 1 mM ouabain, there was an increase in the fraction of slowly diffusing water. The ADCs of the two fractions did not change. These data support the hypothesis that the decrease in the ADC of brain water following an ischemic attack is caused by cell swelling. The relative amplitudes of the two diffusing fractions do not match the expected ratio of intracellular and extracellular fractions. This discrepancy may be principally due to the difference in T2 relaxation rates of the two compartments.
AB - High resolution NMR imaging of the isolated perfused rat hippocampal slice was used to quantitate ADC changes following ouabain-induced cell swelling. Hippocampal slices were studied in artificial cerebrospinal fluid and then in ouabain using a 600-MHz narrow bore spectrometer and a home- built perfusion chamber. The brain slices demonstrated biexponential diffusion behavior. After perfusion with 1 mM ouabain, there was an increase in the fraction of slowly diffusing water. The ADCs of the two fractions did not change. These data support the hypothesis that the decrease in the ADC of brain water following an ischemic attack is caused by cell swelling. The relative amplitudes of the two diffusing fractions do not match the expected ratio of intracellular and extracellular fractions. This discrepancy may be principally due to the difference in T2 relaxation rates of the two compartments.
KW - Ischemia
KW - NMR imaging
KW - Ouabain
KW - Water diffusion
UR - http://www.scopus.com/inward/record.url?scp=0032903849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032903849&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1522-2594(199901)41:1<137::AID-MRM19>3.0.CO;2-Y
DO - 10.1002/(SICI)1522-2594(199901)41:1<137::AID-MRM19>3.0.CO;2-Y
M3 - Article
C2 - 10025621
AN - SCOPUS:0032903849
VL - 41
SP - 137
EP - 142
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
SN - 0740-3194
IS - 1
ER -