The effect of nucleotides (ADP and ADP + Vi) on the thermal stability of rat uterus

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Abstract

Thermal unfolding of stripes prepared from rat uterus has been studied in the presence of nucleotides by differential scanning calorimetry (DSC). Using ADP, ATP and inorganic phosphate analogue orthovanadate, three intermediate states of the ATP hydrolysis cycle were simulated in the uterus stripes. In the main transition of the DSC pattern at least four overlapping endotherms were detected in rigor (AM), in strongly binding (AM · ADP) and weakly binding state (AM · ADP · Vi) of myosin to actin. It was found that nucleotide binding induced a shift of the main melting temperatures (from 60.7 to 61.1°C) and produced changes in the total calorimetric enthalpies (0.45 J/g for rigor, 0.4 J/g for strong binding, and 0.6 J/g for weak binding state). In the Krebs-Ringer bicarbonate buffer containing 100 nM estrogen (Oe) the main transition temperature shifted to 62.4°C and the total enthalpy change was 0.56 J/g. It seems to be an intermediate phase between the strong and weak binding state. The changes of the parameters of the peak functions suggest global rearrangements of the internal structure in myosin heads in the intermediate states.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalThermochimica Acta
Volume376
Issue number2
DOIs
Publication statusPublished - Sep 7 2001

Fingerprint

uterus
Administrative data processing
adenosine diphosphate
nucleotides
Nucleotides
Adenosine Diphosphate
rats
Rats
Thermodynamic stability
thermal stability
Adenosinetriphosphate
Myosins
Differential scanning calorimetry
Enthalpy
Adenosine Triphosphate
myosins
Vanadates
adenosine triphosphate
Bicarbonates
Superconducting transition temperature

Keywords

  • ADP
  • ADP + V
  • DSC
  • Smooth muscle
  • Thermal stability
  • Uterus

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

The effect of nucleotides (ADP and ADP + Vi) on the thermal stability of rat uterus. / Lőrinczy, D.; Vértes, Zs; Belágyi, J.

In: Thermochimica Acta, Vol. 376, No. 2, 07.09.2001, p. 109-115.

Research output: Contribution to journalArticle

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