Studying the gelation of Ehrlich ascites tumor cell extracts at various methyglyoxal concentrations, an increase of the gelled protein fraction, composed mainly of actin, was found at 10-7 M to 10-5 M. When methylglyoxal was added to intact tumor cells, the filamentous portion of cytoplasmic actin was increased at 10-7 M to 10-6 M concentrations. Furthermore, certain functional properties of purified skeletal muscle actin were also affected by μM concentrations of methylglyoxal; the speed of actin polymerization was facilitated and more filamentous actin formed in polymerizing conditions. The possible mode of methylglyoxal action is discussed.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - Mar 31 1981|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology