The effect of light chain gene expression on the inheritance of an idiotype associated with primary anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies

T. Imanishi-Kari, E. Rajnavölgyi, T. Takemori, R. S. Jack, K. Rajewsky

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Abstract

Primary anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies from C57BL/6 mice were idiotypically defined by a rabbit anti-idiotypic antiserum. The idiotypic marker detected by this antiserum (the NPb idiotype) requires for its expression a specific heavy (H) chain-lambda (λ) light (L) chain combination as shown in chain reassociation experiments. The idiotypic binding reaction is inhibitable by hapten. BALB/c and CBA mice also produce large amounts of λ-bearing anti-NP antibodies, but these do not express the NPb idiotype as defined by the rabbit antiserum. Since the NPb idiotype could be reconstituted by reassociating C57BL/6 anti-NP antibody H chains with either HOPC 1, or BALB/c or CBA anti-NP antibody λ chains, the absence of the NPb idiotype in BALB/c and CBA antibodies implies that these animals express different sets of H chain variable (V) regions in the anti-NP response. SJL mice, in contrast to other mouse strains with the Ig-1b allotype, express only traces of NPb idiotype in their anti-NP antibodies, the L chains of which are almost exclusively of the χ type. Knowing that SJL mice possess a regulatory gene responsible for the low level of λ (λ(lo)) chains in their immunoglobulins, we have analyzed the progeny of (SJL x BALB/c)F1, (SJL x BALB/c)F1 x BALB/c and (SJL x BALB/c)F1 x SJL for the expression of λ-bearing anti-NP antibodies and NPb idiotype. In accord with previous work (SJL x BALB/c)F1 mice produced substantial amounts of anti-NP antibodies with the NPb idiotype. Backcross of these mice to BALB/c revealed linkage of the NPb idiotype to the Ig-1b allotype, in agreement with previous data. Backcross of the F1 animals to SJL demonstrated control of the NPb idiotype by the gene regulating λ chain expression in serum immunoglobulin. Chain reassociation experiments showed that in SJL mice, the regulation against λ chain expression prevents the selection in the anti-NP response of those H chains that together with λ chains constitute the NPb idiotype. Apparently, these H chains are unable to contribute to a binding site with specificity for the NP hapten when combined with χ chains.

Original languageEnglish
Pages (from-to)324-331
Number of pages8
JournalEuropean Journal of Immunology
Volume9
Issue number4
DOIs
Publication statusPublished - 1979

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Anti-Idiotypic Antibodies
Gene Expression
Light
Antibodies
Immunoglobulin Allotypes
Immune Sera
Immunoglobulins
Rabbits
Inbred CBA Mouse
Haptens
Regulator Genes
Inbred C57BL Mouse
4-hydroxy-5-nitrophenyl acetic acid
Binding Sites
Serum
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

The effect of light chain gene expression on the inheritance of an idiotype associated with primary anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies. / Imanishi-Kari, T.; Rajnavölgyi, E.; Takemori, T.; Jack, R. S.; Rajewsky, K.

In: European Journal of Immunology, Vol. 9, No. 4, 1979, p. 324-331.

Research output: Contribution to journalArticle

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abstract = "Primary anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies from C57BL/6 mice were idiotypically defined by a rabbit anti-idiotypic antiserum. The idiotypic marker detected by this antiserum (the NPb idiotype) requires for its expression a specific heavy (H) chain-lambda (λ) light (L) chain combination as shown in chain reassociation experiments. The idiotypic binding reaction is inhibitable by hapten. BALB/c and CBA mice also produce large amounts of λ-bearing anti-NP antibodies, but these do not express the NPb idiotype as defined by the rabbit antiserum. Since the NPb idiotype could be reconstituted by reassociating C57BL/6 anti-NP antibody H chains with either HOPC 1, or BALB/c or CBA anti-NP antibody λ chains, the absence of the NPb idiotype in BALB/c and CBA antibodies implies that these animals express different sets of H chain variable (V) regions in the anti-NP response. SJL mice, in contrast to other mouse strains with the Ig-1b allotype, express only traces of NPb idiotype in their anti-NP antibodies, the L chains of which are almost exclusively of the χ type. Knowing that SJL mice possess a regulatory gene responsible for the low level of λ (λ(lo)) chains in their immunoglobulins, we have analyzed the progeny of (SJL x BALB/c)F1, (SJL x BALB/c)F1 x BALB/c and (SJL x BALB/c)F1 x SJL for the expression of λ-bearing anti-NP antibodies and NPb idiotype. In accord with previous work (SJL x BALB/c)F1 mice produced substantial amounts of anti-NP antibodies with the NPb idiotype. Backcross of these mice to BALB/c revealed linkage of the NPb idiotype to the Ig-1b allotype, in agreement with previous data. Backcross of the F1 animals to SJL demonstrated control of the NPb idiotype by the gene regulating λ chain expression in serum immunoglobulin. Chain reassociation experiments showed that in SJL mice, the regulation against λ chain expression prevents the selection in the anti-NP response of those H chains that together with λ chains constitute the NPb idiotype. Apparently, these H chains are unable to contribute to a binding site with specificity for the NP hapten when combined with χ chains.",
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AU - Rajewsky, K.

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N2 - Primary anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies from C57BL/6 mice were idiotypically defined by a rabbit anti-idiotypic antiserum. The idiotypic marker detected by this antiserum (the NPb idiotype) requires for its expression a specific heavy (H) chain-lambda (λ) light (L) chain combination as shown in chain reassociation experiments. The idiotypic binding reaction is inhibitable by hapten. BALB/c and CBA mice also produce large amounts of λ-bearing anti-NP antibodies, but these do not express the NPb idiotype as defined by the rabbit antiserum. Since the NPb idiotype could be reconstituted by reassociating C57BL/6 anti-NP antibody H chains with either HOPC 1, or BALB/c or CBA anti-NP antibody λ chains, the absence of the NPb idiotype in BALB/c and CBA antibodies implies that these animals express different sets of H chain variable (V) regions in the anti-NP response. SJL mice, in contrast to other mouse strains with the Ig-1b allotype, express only traces of NPb idiotype in their anti-NP antibodies, the L chains of which are almost exclusively of the χ type. Knowing that SJL mice possess a regulatory gene responsible for the low level of λ (λ(lo)) chains in their immunoglobulins, we have analyzed the progeny of (SJL x BALB/c)F1, (SJL x BALB/c)F1 x BALB/c and (SJL x BALB/c)F1 x SJL for the expression of λ-bearing anti-NP antibodies and NPb idiotype. In accord with previous work (SJL x BALB/c)F1 mice produced substantial amounts of anti-NP antibodies with the NPb idiotype. Backcross of these mice to BALB/c revealed linkage of the NPb idiotype to the Ig-1b allotype, in agreement with previous data. Backcross of the F1 animals to SJL demonstrated control of the NPb idiotype by the gene regulating λ chain expression in serum immunoglobulin. Chain reassociation experiments showed that in SJL mice, the regulation against λ chain expression prevents the selection in the anti-NP response of those H chains that together with λ chains constitute the NPb idiotype. Apparently, these H chains are unable to contribute to a binding site with specificity for the NP hapten when combined with χ chains.

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