The effect of lentinan on artificial metastasis has been studied in lung-colony assay using low and highly metastatic strains of Lewis lung cancer. Lentinan pretreatment of the tumour-bearing hosts decreased the number of colonies in the lungs in a dose-dependent way in the case of the low metastatic, LLT strain. The same treatment, on the other hand, was ineffective against a highly metastatic variant of the same tumour developed by in vivo selection. The possible cause of the unresponsiveness of the selected highly metastatic strain to the lentinan treatment has been considered in the light of its altered biological features revealed in earlier studies. The increased metastatic capacity achieved by the in vivo selection procedure was accompanied by several phenotypic alterations. The highly metastatic variant was characterized by an elevated glycosaminoglycan (GAG)m content on the cell surface with a shift to a higher amount of heparan sulfate at the expense of chondroitin sulfate, and its cells expressed Class 1 antigens of the major histocompatibility complex (MHC) and showed decreased in vivo sensitivity to macrophages and NK cells. It is suggested that the unresponsiveness to lentinan might be due to these membrane changes.
|Number of pages||7|
|Journal||International Journal of Immunotherapy|
|Publication status||Published - Dec 1 1989|
ASJC Scopus subject areas
- Immunology and Allergy