The effect of L-arginine/nitric oxide pathway on salivary amylase secretion in conscious rats

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In a recent study we have demonstrated the presence of nitric oxide synthase immunoreactive neurons and also perivascular periacinar and periductal nerve fibres in feline submandibular salivary gland. The role of nitric oxide (NO) in salivary vasoregulation has been suggested by other data too, but the effect of NO on salivary amylase secretion has not been investigated yet. Under ether anesthesia a catheter was introduced into the oesophagus for salivary juice collections, and a cannula was inserted into the jugular vein for infusions. After postanaesthesia recovery, submaximal carbachol infusion was given as a background to obtain steady secretion because of the low basal secretory rate. Then different groups of animals received NO synthase inhibitor NOLA (N(G)-nitro L-arginine), L-arginine, D-arginine or NO donor SIN-1 (3-morpholinosydnonimine). Volume and amylase activity were determined in mixed saliva samples collected for 30 min. Carbachol background infusion alone induced an elevated, sustained salivary secretion. NOLA (30 mg/kg) increased both volume and amylase output (P <0.001). This effect was prevented by L-arginine but not by D-arginine. SIN-1 did not change either volume or amylase secretion. The present results suggest that the L-arginine/NO pathway has a modulatory effect on salivary fluid and amylase secretion, which is probably nor related to its effect on salivary blood flow. NO may block certain presently unidentified secretagogue mechanisms and/or may relax myoepithelial cells.

Original languageEnglish
Pages (from-to)217-221
Number of pages5
JournalJournal of Physiology Paris
Volume91
Issue number3-5
DOIs
Publication statusPublished - May 1997

Fingerprint

Amylases
Arginine
Nitric Oxide
Carbachol
Nitric Oxide Synthase
Secretory Rate
Fluids and Secretions
Nitric Oxide Donors
Submandibular Gland
Felidae
Jugular Veins
Salivary Glands
Nerve Fibers
Saliva
Ether
Esophagus
Catheters
Anesthesia
Neurons

Keywords

  • Amylase
  • Exocrine
  • Fluid secretion
  • L-arginine
  • Nitric oxide
  • Rat
  • Saliva

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

Cite this

@article{62aff21b46f245e7922b10af8b249e3d,
title = "The effect of L-arginine/nitric oxide pathway on salivary amylase secretion in conscious rats",
abstract = "In a recent study we have demonstrated the presence of nitric oxide synthase immunoreactive neurons and also perivascular periacinar and periductal nerve fibres in feline submandibular salivary gland. The role of nitric oxide (NO) in salivary vasoregulation has been suggested by other data too, but the effect of NO on salivary amylase secretion has not been investigated yet. Under ether anesthesia a catheter was introduced into the oesophagus for salivary juice collections, and a cannula was inserted into the jugular vein for infusions. After postanaesthesia recovery, submaximal carbachol infusion was given as a background to obtain steady secretion because of the low basal secretory rate. Then different groups of animals received NO synthase inhibitor NOLA (N(G)-nitro L-arginine), L-arginine, D-arginine or NO donor SIN-1 (3-morpholinosydnonimine). Volume and amylase activity were determined in mixed saliva samples collected for 30 min. Carbachol background infusion alone induced an elevated, sustained salivary secretion. NOLA (30 mg/kg) increased both volume and amylase output (P <0.001). This effect was prevented by L-arginine but not by D-arginine. SIN-1 did not change either volume or amylase secretion. The present results suggest that the L-arginine/NO pathway has a modulatory effect on salivary fluid and amylase secretion, which is probably nor related to its effect on salivary blood flow. NO may block certain presently unidentified secretagogue mechanisms and/or may relax myoepithelial cells.",
keywords = "Amylase, Exocrine, Fluid secretion, L-arginine, Nitric oxide, Rat, Saliva",
author = "Zs. Lohinai and B. Burghardt and T. Zelles and G. Varga",
year = "1997",
month = "5",
doi = "10.1016/S0928-4257(97)89488-0",
language = "English",
volume = "91",
pages = "217--221",
journal = "Journal de Physiologie",
issn = "0928-4257",
publisher = "Elsevier Masson SAS",
number = "3-5",

}

TY - JOUR

T1 - The effect of L-arginine/nitric oxide pathway on salivary amylase secretion in conscious rats

AU - Lohinai, Zs.

AU - Burghardt, B.

AU - Zelles, T.

AU - Varga, G.

PY - 1997/5

Y1 - 1997/5

N2 - In a recent study we have demonstrated the presence of nitric oxide synthase immunoreactive neurons and also perivascular periacinar and periductal nerve fibres in feline submandibular salivary gland. The role of nitric oxide (NO) in salivary vasoregulation has been suggested by other data too, but the effect of NO on salivary amylase secretion has not been investigated yet. Under ether anesthesia a catheter was introduced into the oesophagus for salivary juice collections, and a cannula was inserted into the jugular vein for infusions. After postanaesthesia recovery, submaximal carbachol infusion was given as a background to obtain steady secretion because of the low basal secretory rate. Then different groups of animals received NO synthase inhibitor NOLA (N(G)-nitro L-arginine), L-arginine, D-arginine or NO donor SIN-1 (3-morpholinosydnonimine). Volume and amylase activity were determined in mixed saliva samples collected for 30 min. Carbachol background infusion alone induced an elevated, sustained salivary secretion. NOLA (30 mg/kg) increased both volume and amylase output (P <0.001). This effect was prevented by L-arginine but not by D-arginine. SIN-1 did not change either volume or amylase secretion. The present results suggest that the L-arginine/NO pathway has a modulatory effect on salivary fluid and amylase secretion, which is probably nor related to its effect on salivary blood flow. NO may block certain presently unidentified secretagogue mechanisms and/or may relax myoepithelial cells.

AB - In a recent study we have demonstrated the presence of nitric oxide synthase immunoreactive neurons and also perivascular periacinar and periductal nerve fibres in feline submandibular salivary gland. The role of nitric oxide (NO) in salivary vasoregulation has been suggested by other data too, but the effect of NO on salivary amylase secretion has not been investigated yet. Under ether anesthesia a catheter was introduced into the oesophagus for salivary juice collections, and a cannula was inserted into the jugular vein for infusions. After postanaesthesia recovery, submaximal carbachol infusion was given as a background to obtain steady secretion because of the low basal secretory rate. Then different groups of animals received NO synthase inhibitor NOLA (N(G)-nitro L-arginine), L-arginine, D-arginine or NO donor SIN-1 (3-morpholinosydnonimine). Volume and amylase activity were determined in mixed saliva samples collected for 30 min. Carbachol background infusion alone induced an elevated, sustained salivary secretion. NOLA (30 mg/kg) increased both volume and amylase output (P <0.001). This effect was prevented by L-arginine but not by D-arginine. SIN-1 did not change either volume or amylase secretion. The present results suggest that the L-arginine/NO pathway has a modulatory effect on salivary fluid and amylase secretion, which is probably nor related to its effect on salivary blood flow. NO may block certain presently unidentified secretagogue mechanisms and/or may relax myoepithelial cells.

KW - Amylase

KW - Exocrine

KW - Fluid secretion

KW - L-arginine

KW - Nitric oxide

KW - Rat

KW - Saliva

UR - http://www.scopus.com/inward/record.url?scp=0030719494&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030719494&partnerID=8YFLogxK

U2 - 10.1016/S0928-4257(97)89488-0

DO - 10.1016/S0928-4257(97)89488-0

M3 - Article

C2 - 9403798

AN - SCOPUS:0030719494

VL - 91

SP - 217

EP - 221

JO - Journal de Physiologie

JF - Journal de Physiologie

SN - 0928-4257

IS - 3-5

ER -