The effect of kisspeptin on the regulation of vascular tone

Zsófia Mezei, Omid Zamani-Forooshani, Krisztina Csabafi, Bence Szikszai, Eszter Papp, Ádám Ónodi, Dóra Török, Ádám Leprán, Gyula Telegdy, Gyula Szabó

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1 Citation (Scopus)

Abstract

Kisspeptin has been implicated in cardiovascular control. Eicosanoids play a crucial role in the activation of platelets and the regulation of vascular tone. In the present study, we investigated the effect of kisspeptins on eicosanoid synthesis in platelets and aorta in vitro. Platelets and aorta were isolated from Wistar–Kyoto rats. After preincubation with different doses of kisspeptin, samples were incubated with [1-14C]arachidonic acid (0.172 pmol/mL) in tissue culture Medium 199. The amount of labeled eicosanoids was measured with liquid scintillation, after separation with overpressure thin-layer chromatography. Kisspeptin-13 stimulated the thromboxane synthesis. The dose–response curve was bell-shaped and the most effective concentration was 2.5 × 10−8 mol/L, inducing a 27% increase. Lipoxygenase products of platelets displayed a dose-dependent elevation up to the dose of 5 × 10−8 mol/L. In the aorta, kisspeptin-13 induced a marked elevation in the production of 6-keto-prostaglandin F, the stable metabolite of prostacyclin, and lipoxygenase products. Different effects of kisspeptin on cyclooxygenase and lipoxygenase products indicate that beyond intracellular Ca2+ mobilization, other signaling pathways might also contribute to its actions. Our data suggest that kisspeptin, through the alteration of eicosanoid synthesis in platelets and aorta, may play a physiologic and (or) pathologic role in the regulation of vascular tone.

Original languageEnglish
Pages (from-to)787-791
Number of pages5
JournalCanadian journal of physiology and pharmacology
Volume93
Issue number9
DOIs
Publication statusPublished - Apr 15 2015

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Keywords

  • Aorta
  • Eicosanoids
  • Kisspeptin
  • Platelets

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Mezei, Z., Zamani-Forooshani, O., Csabafi, K., Szikszai, B., Papp, E., Ónodi, Á., Török, D., Leprán, Á., Telegdy, G., & Szabó, G. (2015). The effect of kisspeptin on the regulation of vascular tone. Canadian journal of physiology and pharmacology, 93(9), 787-791. https://doi.org/10.1139/cjpp-2015-0013