The effect of juglone on the membrane potential and whole-cell K+ currents of human lymphocytes

Zoltán Varga, László Bene, Carlo Pieri, Sándor Damjanovich, Rezso Gáspár

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Using flow cytometric membrane potential measurements with the oxonol dye, we determined that 5.7-57 μM juglone depolarizes human lymphocytes in a dose dependent manner. The depolarizing effect of juglone was verified by patch-clamp. Juglone decreased whole-cell n-type K+ currents in human peripheral blood lymphocytes and accelerated inactivation; however, it did not influence the kinetics of activation of the K+ conductance. The percentage increase in K+ channel inactivation rate and the degree of drug induced block was independent of membrane potential. K+ channel block by jugalone fully developed within 4 minutes and was not removable by washing with drug free extracellular solution. Blocking of n-type K+ channels by juglone is in concert with its depolarizing effect on human lymphocytes.

Original languageEnglish
Pages (from-to)828-832
Number of pages5
JournalBiochemical and biophysical research communications
Volume218
Issue number3
DOIs
Publication statusPublished - Jan 26 1996

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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